S5545

Anti-Serotonin antibody produced in rabbit

whole antiserum

• Synonym(s): Anti-Serotonin, Rabbit Anti-Serotonin, Serotonin Detection Antibody
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD00162839
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC (f), IHC (p)
• Citations: 276

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: whole antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• contains: 15 mM sodium azide
• species reactivity: rat, human
• packaging: antibody small pack of 25 μL
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:8,000 using sections of normal human appendix or serotonin-containing carcinoid tumors, immunohistochemistry (frozen sections): 1:5,000 using perfusion-fixed (4% paraformaldehyde), frozen sections of rat brain.
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HTR1A(3350)

Description

General description

Serotonin/5-HT (5-hydroxytryptamine) is a neurotransmitters present in the brain. Anti-Serotonin specifically stains enterochromaffin cells in formalin-fixed, paraffin-embedded sections of normal human appendix and serotonin-containing carcinoid tumors. In the central nervous system (CNS), the antiserum reacts with serotonin-containing fibers in perfusion-fixed, free-floating sections of rat brain.

Immunogen

Serotonin creatinine sulfate complex conjugated with formaldehyde to BSA.

Application

Anti-Serotonin antibody has been used in immunocytochemistry.

Biochem/physiol Actions

Serotonin/5-HT, the midbrain raphe system controls mood, emotion, cognition and motor functions, circadian and neuroendocrine rhythms, like food intake, sleep and reproductive activity. It participates in natural reward-related physiology and behaviour, from feeding to sexual activity.

The antibody reacts with serotonin-containing fibers in paraformaldehyde perfusion-fixed, frozen sections of rat brain. The product specifically stains enterochromaffin cells in formalin-fixed, paraffin embedded sections of normal human appendix and serotonin-containing carcinoid tumors.

Physical form

Supplied as a liquid containing 15 mM sodium azide as preservative.

Preparation Note

For continuous use, store at 2-8 °C for a maximum of one month. For extended storage freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable