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Sucrose Phosphorylase

Name: Sucrose Phosphorylase
Brand: SIGMA

recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid

Synonym(s):

SPase, disaccharide glucosyltransferase, sucrose glucosyltransferase, Sucrose:orthophosphate α-D-glucosytransferase

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About This Item

CAS Number:

9074-06-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

2.4.1.7 (BRENDA, IUBMB)

MDL number:

MFCD00132397

Specific activity:

≥45 units/mg solid

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Segment

200

form

lyophilized powder

specific activity

≥45 units/mg solid

mol wt

56 kDa by SDS-PAGE

shipped in

wet ice

storage temp.

−20°C

Description

General description

Research area: Cell signaling
Sucrose Phosphorylase belongs to glycoside hydrolase, GH13 family. It comprises of four domains with the glucose anomeric carbon-binding site and a glucoside-binding site. The active site residues include Asp192 and Glu232. It is majorly produced by bifidobacteria and lactic acid bacteria. The cross-linked sucrose phosphorylase aggregates is thermostable and could be exploited for industrial catalysis of glycosylation.

Application

Sucrose Phosphorylase has been used in sucrose determination in wheat plant and in sucrose hydrogen production.

Sucrose phosphorylase has been used:

To assess the enzymatic synthesis of stable, odorless, and powdered furanone glucosides.
To investigate the novel transglucosylating reaction with carboxylic compounds.
In sucrose determination in wheat plant and in sucrose hydrogen production.

Biochem/physiol Actions

Sucrose phosphorylase catalyzes the reversible conversion of sucrose (α-D-glucopyranosyl-1,2-β-D-fructofuranoside) and phosphate into D-fructose and α-D-glucose 1-phosphate. This reaction plays a crucial role in generating the vital glucose component through sucrose metabolism.(1)

Physical form

Contains sucrose as stabilizer.

Other Notes

One unit will produce 1.0 μmole of D-fructose from sucrose per min with the corresponding reduction of NADP to NADPH at pH 7.6, at 25 °C.

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This Item	16104	718033	721891
Sigma-Aldrich S0937 Sucrose Phosphorylase	Sigma-Aldrich 16104 Sucrose	Sigma-Aldrich 718033 Sucrose solution	Supelco 721891 Sucrose solution
description recombinant, expressed in E. coli, lyophilized powder, ≥45 units/mg solid	description puriss., meets analytical specification of Ph. Eur., BP, NF	description suitable for NMR (reference standard), 2 mM in H₂O:D₂O (9:1) (99.9 atom % D), sodium azide (trace), sodium chloride 0.25 M	description suitable for NMR (reference standard), 2 mM in H₂O:D₂O (9:1) (99.9 atom % D), DSS 0.5 mM, NMR tube size 3 mm × 8 in.
specific activity ≥45 units/mg solid	specific activity -	specific activity -	specific activity -
recombinant expressed in E. coli	recombinant -	recombinant -	recombinant -
form lyophilized powder	form solid	form solution	form -
shipped in wet ice	shipped in -	shipped in -	shipped in -
storage temp. −20°C	storage temp. -	storage temp. -	storage temp. -
mol wt 56 kDa by SDS-PAGE	mol wt -	mol wt -	mol wt -

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

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