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RAB0648

Human D-Dimer ELISA Kit

for serum, plasma, cell culture supernatants and urine

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About This Item

NACRES:
NA.84
UNSPSC Code:
41116158
Gene information:
human ... FGA(2243)
Input:
sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
Pricing and availability is not currently available.
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.08 pg/mL
standard curve range: 0.082-60 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FGA(2243)

General description

This ELISA kit provides for the quantitative measurement of human D-Dimer in cell culture supernatants, plasma and serum.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

D-Dimer is a fibrin degradation product. It facilitates the activation of the coagulation system. Overexpression of the D-dimer contributes to endothelial dysfunction in virologically suppressed, human immunodeficiency virus (HIV) -infected adults. In addition, increased expression of the gene also serves as a potent predictive biomarker, in patients at risk for disseminated intravascular coagulation (DIC).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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RAB0738RAB0460RAB0511
description

for serum, plasma, cell culture supernatants and urine

description

-

description

for serum, plasma, and cell culture supernatants

description

for serum, plasma and cell culture supernatant

Gene Information

human ... FGA(2243)

Gene Information

human ... TGFBR3(7049)

Gene Information

human ... TGFB1(7040)

Gene Information

rat ... Vegfa(83785)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

rat

input

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.08 pg/mL
standard curve range: 0.082-60 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 40 pg/mL
standard curve range: 40.96-10000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 80 pg/mL
standard curve range: 0.0823-60 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 0.82-200 pg/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

Pictograms

Corrosion

Signal Word

Warning

Hazard Codes

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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Certificates of Analysis (COA)

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Questions

1–2 of 2 Questions  
  1. RAB0648 Sigma-Aldrich Human D-Dimer ELISA Kit Does this cross-react with monkey (cyno/NHP)?

    1 answer
    1. This kit has not been tested for suitability in monkey. It is recommended that the end user run a protein BLAST to compare homology between species. For ELISA, if there is a >60% homology, it is likely the kit can work for NHP samples.

      Helpful?

  2. What anticoagulants can be used to collect plasma samples for the Human D-Dimer ELISA (RAB0648)?

    1 answer
    1. The Human D-Dimer ELISA (RAB0648) has been validated with plasma samples treated with EDTA, Citrate, and Sodium heparin.

      Helpful?

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