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RAB0556

Human VWF / von Willebrand Factor ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonym(s):

VWF ELISA Kit

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About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... VWF(7450)
Input:
sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
Pricing and availability is not currently available.
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 0.4 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... VWF(7450)

General description

Human VWF/von Willebrand factor ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro assay for the quantitative measurement of a target protein in biological samples, such as serum, plasma, cell culture supernatants, urine and cell and tissue lysates.

Application

Human VWF/von Willebrand factor ELISA kit has been used for the quantification of endothelial marker-von Willebrand factor in bone marrow-derived mesenchymal stem cells (BMSC) and human umbilical vein endothelial cells (HUVEC).
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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RAB1777MABT856F3520
description

for serum, plasma, cell culture supernatants and urine

description

for serum, plasma and cell culture supernatants

description

clone IIIE2.34, from mouse

description

IgG fraction of antiserum, buffered aqueous solution

Gene Information

human ... VWF(7450)

Gene Information

human ... VWF(7450)

Gene Information

human ... VWF(7450)

Gene Information

human ... VWF(7450)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

-

input

-

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 0.4 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.8 ng/mL
standard curve range: 0.819-200 ng/mL

assay range

-

assay range

-

detection method

colorimetric

detection method

colorimetric

detection method

-

detection method

-

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tissue (Indirect Immunoperoxidase Labeling), indirect immunofluorescence: 1:200 using formalin-fixed, paraffin-embedded sections of human tissue


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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Certificates of Analysis (COA)

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Questions

1–2 of 2 Questions  
  1. можно ли применять этот набор для определения в лекарственных препаратах, есть ли ссылка на фармакопею

    1 answer
    1. This kit has been verified for use to measure vWF in serum and plasma samples. It has not been tested for detection of medicinal products or referenced in the pharmacopoeia. It is intended for Research Use Only.

      Helpful?

  2. Sabéis si este Kit podría ser utilizado con muestras de plasma de rata?

    1 answer
    1. The Human VWF ELISA (RAB0556) has not been tested for reactivity to other species, including rats.

      Helpful?

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