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RAB0420

Human Serum Amyloid A ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

SAA, Serum amyloid A-1 protein 

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About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... SAA1(6288)
Input:
sample type cell culture supernatant(s)
sample type urine
sample type plasma
sample type serum
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice
Pricing and availability is not currently available.
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type urine
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 500 pg/mL
standard curve range: 0.41-300 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SAA1(6288)

General description

Serum amyloid A1 (SAA1) is an acute response protein. It is synthesized by the liver, during infection. SAA1 is expressed in the amnion and chorion of human fetal membranes. It is located on human chromosome chromosome 11. SAA1 encodes for the inducible forms of serum amyloid A (SAA) proteins.
The Human SAA (Serum Amyloid A) ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SAA in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human SAA

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Serum amyloid A1 (SAA1) induces the generation of proinflammatory cytokines like, IL-1β (interleukin 1 β), interleukin 6 (IL-6) and the uterotonic hormone proinflammatory cytokines and prostaglandin E2 (PGE2).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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This Item
EZBRAIN40EZBRAIN42RAB0530
description

for serum, plasma, cell culture supernatant and urine

description

-

description

-

description

for serum, plasma, cell culture supernatants and urine

Gene Information

human ... SAA1(6288)

Gene Information

-

Gene Information

human ... APP(351)

Gene Information

human ... APP(351)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

input

sample type cell culture supernatant(s)
sample type urine
sample type plasma
sample type serum

input

-

input

sample type tissue/cellular lysate

input

sample type cell culture supernatant(s)
sample type urine
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 500 pg/mL
standard curve range: 0.41-300 ng/mL

assay range

-

assay range

sensitivity: 8 pg/mL
(lowest level of Amyloid β1-42 standard; 50 μL sample size), standard curve range: 16-500 pg/mL

assay range

inter-assay cv: <10%
intra-assay cv: <12%
sensitivity: 0.1 ng/mL

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric (450nm/590nm)

detection method

colorimetric

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    SDS

  • ELISA 1X Assay/Sample Diluent Buffer C (Item L)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

Storage Class

10 - Combustible liquids

Pictograms

Health hazard

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1



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Certificates of Analysis (COA)

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Questions

1–2 of 2 Questions  
  1. Could you please let me know whether this assay has been evaluated for interference from rheumatoid factor (RF) and other heterophilic antibodies? If available, I would appreciate any validation data or documentation.

    1 answer
    1. This assay has not been tested for interference from rheumatoid factor or other heterophilic antibodies.

      Helpful?

  2. Has RAB0420- Human Serum Amyloid A ELISA Kit (ELH-SAA-1) been tested with rat sample ?

    1 answer
    1. Unfortunately, the RAB0420 kit has not been tested on rat serum. The cross reactivity has not been investigated and suitability would have to be determined by the end user.

      Helpful?

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