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RAB0386

Brain Natriuretic Peptide EIA Kit

for serum, plasma, culture supernatant and cell lysates

Synonym(s):

Gamma-brain natriuretic peptide, Natriuretic peptides B , BNP

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About This Item

NACRES:
NA.32
UNSPSC Code:
41123300
Pricing and availability is not currently available.
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species reactivity

human, mouse, rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, enzyme immunoassay: suitable

input

sample type serum
sample type culture supernatant(s)
sample type plasma
sample type cell lysate

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.66 pg/mL
standard curve range: 0.1–1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... NPPB(4879)

General description

The BNP Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting BNP peptide based on the principle of Competitive Enzyme Immunoassay.

Immunogen

Brain Natriuretic Peptide, synthetic peptide

Application

For research use only. Not for use in diagnostic procedures.
Brain natriuretic peptide EIA Kit has been used to measure brain natriuretic peptide (BNP) secreted in human neonatal ventricular cardiomyocyte culture.

Biochem/physiol Actions

Brain natriuretic peptide (BNP) expressed in mammalian brain and atrial natriuretic peptide (ANP) facilitates various physiological functions including regulation of blood pressure and cardiac remodeling. BNP acts as a potential biomarker of high left ventricular end-diastolic pressure in patients with symptomatic left ventricular (LV) dysfunction. Elevated expression of BNP is observed in acute myocardial infarction (AMI) patients. BNP is also implicated in several biological functions such as diuresis, natriureis, hypotensive action and inhibition of aldosterone secretion.

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This Item
RAB0630RAB0726RAB0385
description

for serum, plasma, culture supernatant and cell lysates

description

for serum, plasma, cell culture supernatants and urine

description

for serum, plasma, cell culture supernatants and urine

description

for serum, plasma, culture supernatant and cell lysates

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

species reactivity

human, mouse, rat

species reactivity

human

species reactivity

human

species reactivity

mouse, human, rat

technique(s)

enzyme immunoassay: suitable, ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

enzyme immunoassay: suitable, ELISA: suitable

Gene Information

human ... NPPB(4879)

Gene Information

human ... NPPB(4879)

Gene Information

human ... NPPB(4879)

Gene Information

human ... NPPA(4878)

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.66 pg/mL
standard curve range: 0.1–1000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 14 pg/mL
standard curve range: 15.63-1000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.14 ng/mL
standard curve range: 0.137-100 ng/mL

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 1.02 pg/mL
standard curve range: 0.1-1000 pg/mL

input

sample type serum
sample type culture supernatant(s)
sample type plasma
sample type cell lysate

input

sample type urine
sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)
sample type urine

input

sample type serum
sample type cell lysate
sample type plasma
sample type culture supernatant(s)


Kit Components Also Available Separately

Product No.
Description
SDS & Pricing

Pictograms

Health hazard

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Storage Class

10 - Combustible liquids

Signal Word

Danger

Hazard Codes

Precautionary Statements

Hazard Classifications

Resp. Sens. 1



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Questions

  1. What is the stability of the anti-BNP antibody concentrate (item N reconstituted with 55 ul of 1X Assay Diluent) from RAB0386 when stored at 4C?

    1 answer
    1. Storing and reusing the reconstituted antibody item N is not recommended as it is not stable when stored. The kit includes 2 vials so that a partial plate may be run.

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