RAB0377

Visfatin EIA Kit

for serum, plasma, culture supernatant and cell lysates

• Synonym(s): Visfatin ELISA Kit
• NACRES: NA.32
• UNSPSC Code: 12352200

Properties

• species reactivity: rat, human, mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, enzyme immunoassay: suitable
• input: sample type serum; sample type culture supernatant(s); sample type cell lysate; sample type plasma
• assay range: inter-assay cv: <15%; intra-assay cv: <10%; sensitivity: 0.778 ng/mL; standard curve range: 0.1-1000 ng/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... NAMPT(10135)

Description

General description

The Visfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Visfatin peptide based on the principle of Competitive Enzyme Immunoassay.

Visfatin, also known as nicotinamide phosphoribosyltransferase (Nampt)/ pre-B Cell colony enhancing factor is encoded by the gene mapped to human chromosome 7q22.3. The gene contains 11 exons encompassing 34.7kb. The encoded protein is an adipocytokine secreted by visceral fat.

Immunogen

Visfatin, synthetic peptide

Application

Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Visfatin EIA Kit has been used to measure the concentration of visfatin in blood samples.

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Visfatin has a role in mammalian nicotinamide adenine dinucleotide (NAD⁺) biosynthesis. Studies have shown that mice deficient in visfatin in the forebrain excitatory neurons show hyperactivity, diminished anxiety-like behaviors and impaired learning and memory. It has also been shown that visfatin stimulates glucose uptake and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation in mouse C2C12 skeletal muscle cells. Visfatin regulates inflammation and insulin sensitivity.

Kit Components Also Available Separately

• RABHRP3

  HRP-Streptavidin

  SDS

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Codes: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 10 - Combustible liquids