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Peroxisome Isolation Kit

Name: Peroxisome Isolation Kit
Brand: SIGMA

isolate peroxisomes from tissues and cells

Synonym(s):

Isolation Kit for Peroxisomes, Kit for Peroxisomes, Peroxisome Kit

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352200

Pricing and availability is not currently available.

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Properties

Quality Segment

200

technique(s)

centrifugation: suitable, fractionation: suitable

input

mammalian sample(s)

shipped in

wet ice

storage temp.

2-8°C

Description

General description

Isolated peroxisomes are used for studying lipid β-oxidation, amino acid metabolism and biosynthesis of ether-linked glycerolipids and bile acids.

Application

The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep^™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.

Features and Benefits

Specially formulated extraction reagents for research scale applications - save time and minimize waste
Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis
Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)

Other Notes

Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

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This Item	LYSISO1	CPISO	GL0010
Sigma-Aldrich PEROX1 Peroxisome Isolation Kit	Sigma-Aldrich LYSISO1 Lysosome Isolation Kit	Sigma-Aldrich CPISO Chloroplast Isolation Kit	Sigma-Aldrich GL0010 Golgi Isolation Kit
description isolate peroxisomes from tissues and cells	description sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells	description isolation of intact chloroplasts from leaves	description sufficient for 50 g (tissue)
technique(s) centrifugation: suitable, fractionation: suitable	technique(s) centrifugation: suitable, fractionation: suitable	technique(s) centrifugation: suitable	technique(s) fractionation: suitable
input mammalian sample(s)	input mammalian sample(s)	input -	input mammalian sample(s)
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in wet ice	shipped in -	shipped in wet ice	shipped in wet ice

Kit Components Also Available Separately

Product No.

Description

SDS & Pricing

P8340
Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
SDS

Pictograms

GHS05

Signal Word

Warning

Hazard Codes

H290,H315,H319

Precautionary Statements

P234 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Flash Point (°F)

188.6 °F - closed cup

Flash Point (°C)

87 °C - closed cup

WGK

WGK 2

Storage Class

10 - Combustible liquids

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