One reference gives a pI of 9.4 for subtilisin Carlsberg. See: Zacharis, E. et al., Proc. Nat. Acad. Sci. USA, 96, 1201-1205 (1999).
P8038
Proteinase, bacterial
Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
Synonym(s):
Proteinase from Bacillus licheniformis, Alkaline Protease, Protease from Bacillus licheniformis, Subtilo peptidase A
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type
Type XXIV
form
lyophilized powder
specific activity
≤0.05 Kunitz units/mg solid RNase
≤5.0 Kunitz units/mg solid DNase
7.0-14.0 units/mg solid
mol wt
27 kDa
purified by
crystallization
shipped in
wet ice
storage temp.
−20°C
Related Categories
Specificity
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Application
The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart.[1] It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA.[2]
This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1[3].
Biochem/physiol Actions
Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .
Physical properties
Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Analysis Note
Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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What is the pI of Product P8038, Proteinase, bacterial?
1 answer-
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What is the pH optimum for Product P8038, Proteinase, bacterial?
1 answer-
Two references cite pH 7.5 as optimal for this enzyme: (a) Ricks et al., Biotechnol. Prog., 8, 197-203 (1992), (b) Doswald et al., Bioorg. Med. Chem., 2, 403-410 (1994).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight (MW) of Product P8038, Proteinase, bacterial?
1 answer-
The enzyme has a MW of 27.5-27.6 kDa. See DeLange & Smith, J. Biol. Chem., 243(9), 2134-2142 (1968).
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How long can I store stock solutions of Product P8038, Proteinase, bacterial?
1 answer-
Our notes indicate that a 0.1% solution (1 mg/mL) in PBS or TBS can be stored for about a week at 0-4°C. For longer term storage, we would advise freezing the stock solution in aliquots.
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