N9891
Monoclonal Anti-Nebulin antibody produced in mouse
clone NB2, ascites fluid
Synonym(s):
Anti-AMC6, Anti-NEB177D, Anti-NEM2
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
NB2, monoclonal
mol wt
antigen 500 kDa
contains
15 mM sodium azide
species reactivity
vertebrates, chicken
technique(s)
indirect immunofluorescence: 1:400 using frozen sections of animal skeletal muscle
western blot: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
chicken ... NEB(101748402)
General description
Nebulin is a 700kDa protein which is expressed in skeletal muscles. It possesses 35 amino acid repeats and a C terminal serine-rich and SH3 domains.
Nebulin is also localized to cardiac muscle. Monoclonal Anti-Nebulin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Specificity
Localizes nebulin in skeletal muscle of a wide variety of species from cold-blooded vertebrates to man. Does not crossreact with smooth or cardiac muscle, non-muscle tissue and cultured cells. Localizes nebulin within the I band of skeletal muscle sections by indirect immunofluorescence.
Immunogen
chicken breast nebulin.
Application
Monoclonal Anti-Nebulin antibody produced in mouse has been used in:
- immunohistochemistry
- fluorescent immunostaining
- western blotting
Biochem/physiol Actions
Nebulin associates with the Z-line and thin actin filament in a sarcomere. It may function as a regulator of sarcomere thin filament length. The protein also acts as a structural link between sarcomeres. Nebulin interacts with the capping protein CapZ and has a role in controlling the localization of actin filaments.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc.
Pappas CT
Molecular Biology of the Cell (2008)
Biallelic mutations in MYPN, encoding myopalladin, are associated with childhood-onset, slowly progressive nemaline myopathy
Miyatake S, et al.
American Journal of Human Genetics, 100(1), 169-178 (2017)
Nebulin interacts with CapZ and regulates thin filament architecture within the Z-disc
Pappas CT, et al.
Molecular Biology of the Cell, 19(5), 1837-1847 (2008)
J Q Zhang et al.
Biophysical journal, 74(1), 349-359 (1998-02-04)
cDNA clones encoding mouse skeletal muscle nebulin were expressed in Escherichia coli as thioredoxin fusion proteins and purified in the presence of 6 M urea. These fragments, called 7a and 8c, contain 28 and 19 of the weakly repeating approximately
A 7.5-kb 3'-Terminal cDNA Sequence of Chicken Skeletal Muscle Nebulin Reveals Its Actin Binding Regions.
Suzuki T
Zoological Science (2000)
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