The product information sheet protocol for this product instructs to grow the cells in the initial treatment media until 80–90% confluency before passaging. If a media change is necessary for cell health, it is important to include the elimination reagent in the new media.
MP0030
LookOut® Mycoplasma Elimination Kit
Synonym(s):
Mycoplasma contamination treatment
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About This Item
NACRES:
NA.75
UNSPSC Code:
12352207
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usage
kit sufficient for 5 mL mycoplasma elimination
Quality Level
packaging
pkg of 1 kit
storage condition
protect from light
technique(s)
PCR: suitable
application(s)
detection
microbiology
storage temp.
2-8°C
1 of 4
This Item | |||
|---|---|---|---|
| technique(s) PCR: suitable | technique(s) PCR: suitable | technique(s) RT-qPCR: suitable | technique(s) PCR: suitable |
| usage kit sufficient for 5 mL mycoplasma elimination | usage sufficient for 25 reactions | usage sufficient for 250 reactions | usage - |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - | packaging pkg of 1 kit |
| storage condition protect from light | storage condition dry at room temperature | storage condition - | storage condition - |
mycoplamsa removal agent, MRA, | mycoplasma detection, qPCR-based assay | - | mycoplasma detection, PCR-based assay |
| application(s) detection | application(s) detection | application(s) - | application(s) detection |
General description
The kit is comprised of a combination of biological agents that reliably and completely eliminate mycoplasma contamination. The initial treatment of this eradication procedure is adequate for mycoplasma elimination in most applications. The second step suppresses and inactivates any remaining mycoplasma using a follow-up antibiotic treatment.
Application
The LookOut® Mycoplasma Elimination Kit has been developed to quickly and efficiently eliminate mycoplasma contamination from cell cultures.
The LookOut® Mycoplasma Elimination Kit is suitable for the elimination of Mollicutes and related organisms (Mycoplasma, Acholeplasma, Spiroplasma, and Entomoplasma) in cell and virus cultures. It is not effective for treating mycoplasma contamination in Chlamydia cultures.
Features and Benefits
- Minimal cytotoxic properties of the reagents
- Highly effective
- Retention of normal cell characteristics
Legal Information
LookOut is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
The product is not intended for use as a biocide under global biocide regulations, including but not limited to US EPA′s Federal Insecticide Fungicide and Rodenticide Act, European Biocidal Products Regulation, Canada’s Pest Management Regulatory Agency, Turkey’s Biocidal Products Regulation, Korea’s Consumer Chemical Products and Biocide Safety Management Act (K-BPR) and others.
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Description
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Storage Class
10 - Combustible liquids
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Find documentation for the products that you have recently purchased in the Document Library.
Optimized PCR-based Detection of Mycoplasma
Dobrovolny PL and Bess D
Journal of Visualized Experiments, 52 (2011)
Related Content
Instructions
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MP0030-1KT | 04061834066828 |
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After I dilute my epithelial cells in the media + primary antibiotic, do I not change the media until they reach 80-90% confluency? I usually change the media on the cells every 2-3 days.
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Has this been tested in primary cell lines (i.e. skeletal muscle) is there any interference with the normal cell physiology?
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The LookOut Mycoplasma Elimination Kit is designed to efficiently eliminate mycoplasma contamination from cell cultures. This kit uses a combination of biological agents to reliably and completely remove mycoplasma contamination. The initial treatment is generally sufficient for mycoplasma elimination in most applications. A second step involves a follow-up antibiotic treatment to suppress and inactivate any remaining mycoplasma. The reagents have minimal cytotoxic properties, and the development of resistant strains is highly unlikely due to the significantly reduced mycoplasma concentration after the initial treatment. Compared to other products for mycoplasma elimination, inactivation, or suppression, the LookOut Mycoplasma Elimination Kit is more effective and has not been shown to cause changes in normal cell characteristics. It is the least cytotoxic method, with overall cytotoxicity demonstrated to be less than 20% across a diverse group of cell lines
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How does the mechanism of action (MOA) of Product No. MP0030, LookOut® Mycoplasma Elimination Kit, differ from that of BM cyclin?
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The key distinction lies in Product No. MP0030, LookOut® Mycoplasma Elimination Kit, which actively eradicates mycoplasma by disrupting the membrane. As a lipopeptide, it identifies cholesterol-rich regions in the membrane (as the concentration of cholesterol in mycoplasma membranes is higher than in eukaryotic cells), creating a pore through which the surrounding medium flows into the mycoplasma, ultimately causing it to burst. In contrast, standard antibiotics simply inhibit metabolic pathways, necessitating the rinsing out of mycoplasma through a medium change. If the process is halted prematurely, any remaining mycoplasma can regrow.
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What antibiotics are included in MP0030, the LookOut® Mycoplasma Elimination Kit? Are fluoroquinolones, macrolides, or tetracyclines part of the kit?
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The MP0030 product contains two mycoplasma-active antibiotics, with one of them being a fluoroquinolone.
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