MBD0055

MediaBoost

non-animal sterile microbial growth supplement

• Synonym(s): growth supplement for germination, microbial culture additive, microbial culture enhancer, microbial media supplement, minimal growth media supplement
• NACRES: NB.21
• UNSPSC Code: 41106212

Properties

• Quality Segment: 200
• sterility: sterile
• form: liquid
• technique(s): microbiological culture: suitable
• shipped in: wet ice
• storage temp.: 2-8°C
• suitability: Bacillus spp., Escherichia spp., Lactobacillus spp., Lactococcus spp., Pseudomonas spp., Saccharomyces spp., bacteria, yeasts

Description

General description

Studying microbial species via cutting edge genomic techniques such as next generation sequencing (NGS) has become increasingly prevalent in recent years; however, genomic methods alone cannot fully replace experimental testing of microbial species. Therefore, the ability to culture microbes in a laboratory setting remains essential for comprehensive study of these communities. Some microbial species can be challenging to culture in standard culture media. Often species of interest or a specific process require specialized media compositions, to provide an optimal environment for maximal proliferation and yield. A few examples includes

• Chemically defined minimal growth media are often used to grow bacteria for proteomics, as a base for stable isotope enrichment in NMR protein structure-function studies, but growth can be limited without the addition of an effective supplement.

• Use of chemically defined minimal growth media in the production of secreted microbial proteins to ensure reproducibility and a higher concentration of the microbial-produced protein of interest.

Application

MediaBoost provides microbial researchers a ready-to-use media supplement to amplify growth in selected gram-positive bacteria, gram-negative bacteria, and yeast species. Application data is available for some of the strains and media combinations that have been tested with MediaBoost, including:

• Bacillus subtilis spores in LB medium
• Lactobacillus rhamnosus in LB medium
• Pseudomonas aeruginosa in M9 medium
• Saccharomyces cerevisiae in YPD, BM2, and IMDM media

Please note strains and media combinations and should be tested on a case-by-case basis by the researcher for experiment optimization.

Features and Benefits

• Boosts Growth of Certain Microorganisms
• Reduces Germination Time
• Ready-Made, Sterile Solution
• Protein-Free

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 3