The assay does work on flat-bottom plates, but round-bottom plates are preferred for incubation in a water bath to ensure even temperature distribution in each well. The suitability may depend on the specific activity of the protein.
It is not recommended to use clear bottom plates for fluorescence-based assays, nor is reading a fluorescent method from the bottom of the plate advisable. The black-walled, clear bottom plates are typically used for visualization with a microscope.
MAK109
LPL Activity Assay Kit
Supplied by Roar Biomedical, Inc.
Synonym(s):
Lipoprotein lipase activity assay kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
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usage
sufficient for 100 fluorometric tests
application(s)
pharmaceutical
detection method
fluorometric
relevant disease(s)
gastrointestinal diseases; cardiovascular diseases
storage temp.
2-8°C
Gene Information
human ... LPL(4023)
General description
Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.
Application
LPL Activity Assay Kit may be used for detection of LPL activity in plasma samples of patients with hypertriglyceridemia.[1]
Suitability
Suitable for the measurement of lipoprotein lipase activity in a variety of tissue samples
Principle
The LPL Activity Assay Kit includes a non-fluorescent substrate emulsion that becomes intensely fluorescent upon interaction with LPL and pre-hydrolyzed substrate for use as a standard to convert the fluorescence intensity reading to moles of reactant formed (λEx=370 nm/λEm=450 nm). The assay is not specific for LPL and will also detect hepatic lipase activity.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
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Protocols
Lipoprotein lipase (LPL) hydrolyzes triglycerides associated with VLDL.
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Which plates are compatible with the MAK109 LPL Activity Assay Kit? Will it work on flat-bottom plates and if the plate has black walls but a clear bottom?
1 answer-
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Is the kit designed for all LPL? Can it provide any selectivity for different types of LPL, such as pancreatic lipase, hepatic lipase, and endothelial lipase?
1 answer-
The substrate could be hydrolyzed by any surface active lipase, offering no selectivity. However, the liquid crystalline physical state of the emulsion provides selectivity for LPL and hepatic lipase (HL). Running post heparin plasma in high salt to inhibit LPL, but not HL, allows for selective activity measurement of LPL or HL. Endothelial lipase (EL) would not be interested in this substrate.
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