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TCA Deblock

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About This Item

NACRES:

NA.21

UNSPSC Code:

41116105

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Properties

Quality Segment

200

type

for DNA synthesis, for DNA synthesis

storage condition

dry at room temperature

concentration

28-32 g/L (TCA content, titration)

impurities

≤200 ppm water content (Karl Fischer)

color

clear

Description

Preparation Note

Trichloroacetic acid / Dichloromethane 3/100 (v/v)

Other Notes

Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

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This Item	L020000	L021000	L023050
Sigma-Aldrich L020000 TCA Deblock	Sigma-Aldrich L020000 TCA Deblock	Sigma-Aldrich L021000 DCA Deblock	Sigma-Aldrich L023050 DCA Deblock
description -	description -	description with Dichloromethane	description configured for ÄKTA^® and OligoPilot^®, with Toluene
color clear	color clear	color clear	color clear
concentration 28-32 g/L (TCA content, titration)	concentration 28-32 g/L (TCA content, titration)	concentration 42.2-51.6 g/L (DCA content, titration)	concentration 42.2-51.6 g/L (DCA content, titration)
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
type for DNA synthesis, for DNA synthesis	type for DNA synthesis	type for DNA synthesis	type for DNA synthesis
impurities ≤200 ppm water content (Karl Fischer)	impurities ≤200 ppm water content (Karl Fischer)	impurities ≤200 ppm water content (Karl Fischer)	impurities ≤200 ppm water content (Karl Fischer)
storage condition dry at room temperature	storage condition dry at room temperature	storage condition dry at room temperature	storage condition dry at room temperature

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Pictograms

GHS08,GHS05,GHS07,GHS09

Signal Word

Danger

Hazard Codes

H315,H318,H335,H336,H351,H411

Precautionary Statements

P202 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system, Respiratory system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point (°F)

212.0 °F - closed cup

Flash Point (°C)

100 °C - closed cup

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