G8404

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥550 units/mg protein (biuret)

• Synonym(s): Entner-Doudoroff enzyme, G6PD, G6PDH, NADP glucose 6-phosphate dehydrogenase, G-6-P-DH
• CAS Number: 9001-40-5
• Enzyme Commission number: 1.1.1.49 (BRENDA, IUBMB)
• MDL number: MFCD00081656
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Leuconostoc mesenteroides)
• Quality Level: 300
• recombinant: expressed in E. coli
• form: ammonium sulfate suspension
• specific activity: ≥550 units/mg protein (biuret)
• mol wt: 128 kDa
• storage condition: (Tightly closed)
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: UPI0021A4D550
• foreign activity: creatine phosphokinase, glutathione reductase, myokinase, NADH oxidase, NADPH oxidase, phosphoglucomutase, 6-phosphogluconic dehydrogenase, phosphoglucose isomerase, lactic dehydrogenase, hexokinase ≤0.01%
• storage temp.: 2-8°C

Description

General description

Glucose 6-phosphate dehydrogenase (G-6-P-DH) is a key regulatory enzyme in the first step of the pentose phosphate pathway.[1] G-6-P-DH is a glycoprotein with a molecular mass of 128 kDa (gel filtration).[2]G6PD is a dimer and consists of a single active site at each subunit.[3]

Research area: Cell Signaling

Application

Glucose-6-phosphate dehydrogenase has been used:

• to test ketose reductase activity in developing maize endosperm.[4]
• to determine the levels of mannose in coronary heart disease patient-derived serum[5]
• to study its activity on extracellular polymeric substance (EPS) extract to determine cell lysis through sonication[6]
• to determine the glucose uptake in cultured human muscle satellite cells[7]

Biochem/physiol Actions

G6PD provides all cells with reducing power as nicotinamide adenine dinucleotide phosphate (NADPH) which enables the cells to balance oxidative stress. Mutations in the G6PD gene are associated with X-linked G6PD deficiency, a hereditary genetic defect such as neonatal jaundice and acute hemolytic anemia.[8]

Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Unit Definition

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ containing 50 mM Tris and 1 mM MgCl₂, pH 7.5

Other Notes

The volume is lot specific and can be calculated using the data:
For example, lot 0000114274
Units per mg Protein = 846
Mg Protein per mL - 9
Units per mL = 7614
G8404-1000U = approx 132 ul

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves