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RPMI-1640 Medium

Name: RPMI-1640 Medium
Brand: SIGMA

HEPES modification, with stable glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

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UNSPSC Code:

12352207

NACRES:

NA.71

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Properties

Product Name

RPMI-1640 Medium, HEPES Modification, With 25 mM HEPES and stable glutamine, liquid, sterile-filtered

Quality Segment

400

sterility

sterile-filtered, sterile

form

liquid

technique(s)

cell culture | mammalian: suitable

components

stable glutamine: yes
phenol red: yes
HEPES: 25 mM
NaHCO₃: yes

shipped in

ambient

storage temp.

2-8°C

Description

Application

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

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This Item	R7513	R1780	R4130
SAFC FG1385 RPMI-1640 Medium	Sigma-Aldrich R7513 RPMI-1640 Medium	Sigma-Aldrich R1780 RPMI-1640 Medium	SAFC R4130 RPMI-1640 Medium
description HEPES Modification, With 25 mM HEPES and stable glutamine, liquid, sterile-filtered	description Modified, with sodium bicarbonate, without methionine, cystine and L-glutamine, liquid, sterile-filtered, suitable for cell culture	description With L-glutamine and sodium bicarbonate. Without arginine, leucine, lysine, and phenol red, liquid, sterile-filtered, suitable for cell culture, designed for isotope labeling for cell culture applications	description HEPES Modification, with L-glutamine and 25mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
form liquid	form liquid	form liquid	form powder
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
sterility sterile-filtered	sterility sterile-filtered	sterility sterile-filtered	sterility -
components stable glutamine: yes phenol red: yes HEPES: 25 mM NaHCO₃: yes	components HEPES: no sodium pyruvate: no phenol red: yes L-glutamine: no NaHCO₃: yes	components NaHCO₃: 2 g/L phenol red: no glucose: 2 g/L (Dextro) L-glutamine: 0.3 g/L	components L-glutamine: yes NaHCO₃: no phenol red: yes HEPES: 25 mM sodium pyruvate: no
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient

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Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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