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F2442

Sigma-Aldrich

Fetal Bovine Serum

Fetal Bovine Serum
1 of 1 reviewers received a sample product or took part in a promotion

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

Synonym(s):

Fetal Bovine Sera, Fetal Calf Serum, FBS, FCS, sera, serum

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71
biological source:
bovine fetus
origin:
USA origin
sterility:
sterile-filtered
shipped in:
dry ice
Pricing and availability is not currently available.

biological source

bovine fetus

Quality Level

500

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting optimal growth-enhancing and growth-suppressing functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media.[1][2] FBS is derived from the calf blood. It is devoid of fibrin and clotting factors. Heat inactivation of FBS blocks the complement system.[3] FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture.[4][5] It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM),[6] Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM),[7] M199[8] and Roswell Park Memorial Institute medium, RPMI-1640.[6]

Application

Fetal Bovine Serum has also been used as a component of:
  • perivascular-resident macrophage-like melanocytes (PVM/M) growth medium[9]
  • synthetic oviduct fluid (SOF)[10]
  • Eagle′s minimum essential medium (EMEM)[11]
  • neurobasal medium[12]
  • Hyclone McCoys 5 A medium[13]
  • Hanks medium[14]

Biochem/physiol Actions

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Other Notes

Tested for the presence of bacteria, virus, mycoplasma, endotoxin and for hemoglobin content. Triple filtered with 0.1 micron membrane under aseptic conditions.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
19G057-A
24B128
0000321240
23A316
23A317

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Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.
Wie SM et al.
The Journal of Biological Chemistry, 289, 10900-10900 (2014)
MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Cunzhi Yu et al.
Frontiers in pharmacology, 8, 832-832 (2017-12-07)
Low solubility, tissue accumulation, and toxicity are chief obstacles to developing triptolide derivatives, so a better understanding of the pharmacokinetics and toxicity of triptolide derivatives will help with these limitations. To address this, we studied pharmacokinetics and toxicity of (5R)-5-hydroxytriptolide
Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods.
van der Valk J et al.
Toxicology in vitro, 24, 1053-1053 (2010)
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Evaluating of Stericup® Quick Release Filtration Devices for Fetal Bovine Serum (FBS) in Cell Culture

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Evaluating of Stericup® Quick Release Filtration Devices for Fetal Bovine Serum (FBS) in Cell Culture

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Evaluating of Stericup® Quick Release Filtration Devices for Fetal Bovine Serum (FBS) in Cell Culture

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Evaluating of Stericup® Quick Release Filtration Devices for Fetal Bovine Serum (FBS) in Cell Culture

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

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Questions

1–10 of 15 Questions  

  1. How to heat inactivate this serum Fetal Bovine Serum USA origin,(Cat Log - F2442)

    1 answer

    1. Heat inactivation occurs when serum is heated to 56°C for a period of 30 minutes.

      Here's a protocol for heat inactivation:
      1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
      2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
      3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
      4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.

      Helpful?

  2. is this Heat Inactivate FBS?

    1 answer

    1. This product has not been heat inactivated.

      Helpful?

  3. can F2442 FBS be used for culturing peritoneal macrophages? what is the suitable one?

    1 answer

    1. This product is tested for suitability in mammalian cell culture. While it is not specifically tested on macrophages, it is subject to stringent specifications, including low endotoxin, and should perform well. Please see the link below to review a sample Certificate of Analysis:
      https://www.sigmaaldrich.com/coa/SIGMA/F2442/23A317

      Helpful?

  4. Can this f2442 fbs be used for culture expansion of mesenchymal stem cells? Please confirm Thanks

    1 answer

    1. Fetal Bovine Serum (Cat. no.: F2442) is suitable for use in mammalian cell culture. However, it is not tested specifically in mesenchymal stem cell culture. There is some evidence in the literature that this product would be suitable. See the link below for a relevant reference:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4545441/

      Helpful?

  5. What is the difference between Fetal Bovine Serum F2442 and 12103C?

    1 answer

    1. Product No. 12103C meets most of the specifications of F2442.  Both products are from the same raw material suppliers and undergo the same stringent testing prior to release. However, the F2442 has been use-tested in hybridoma culture, whereas 12103C has not.

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  6. There is a precipitate in my serum.  Is my serum contaminated?

    1 answer

    1. Precipitates in serum can often appear as large round bodies, even as doublets per microscopic exam. This can be mistaken as a yeast contamination. Brownian motion may be mistaken for motility. These are lipid aggregates that will be readily dissolved when exposed to lipase. Also, these particles will not gram stain or show any growth in culture on aerobic, anaerobic, or fungal media and culture conditions. The performance of the material is not effected. Centrifugation or filtration of small volumes will remove these particles.

      Helpful?

  7. What is the cloning assay performed on Fetal Bovine Serum, Product F2442?

    1 answer

    1. The cloning efficiency assay is performed on each lot of FBS to verify that each lot will support clonal growth of myeloma cells and/or fusion products. The assay is performed using 5% and 10% concentrations of the FBS.

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  8. How is Fetal Bovine Serum, Product F2442, cell culture tested?

    1 answer

    1. Biological performance is assessed using Sigma cell culture medium supplemented with the serum and tested at a final concentration of 10%. Seeding efficiency, doubling time, and final cell density are measured on appropriate cell lines. Growth studies are carried through two subculture generations and cell growth is plotted as a logarithmic function of time in culture. During the testing period, cultures are examined microscopically for atypical morphology and evidence of cytotoxicity. Hybridoma -tested serum has been shown to support mouse myeloma cells and hybridoma cells in culture.

      Helpful?

  9. Is Fetal Bovine Serum, Product F2442, available as a charcoal stripped, de-lipidated serum?

    1 answer

    1. We offer Product No. F6765 (Fetal Bovine Serum, charcoal stripped). The charcoal stripping decreases the levels of hormones; it may also decrease the levels of lipids. If further reduction of lipid levels is required, you may need to treat the serum with fumed silica (e.g. Product No. S5130). Use 20 grams of S5130 per Liter of serum (not plasma). It is mixed for 3-4 hours, then centrifuged in 1 L containers for about 15 minutes at 2000 xg. The supernatant can then be decanted, and filtered if desired. NOTE: Silica is extremely fluffy, and care must be taken not to inhale its dust. Allow for about 15% loss of the serum due to adsorption.

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  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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1–10 of 15 Questions  

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

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  1. 대한민국 서울
    • Review 1
    • Votes 4
    5 out of 5 stars.

    최고의 갓성비 FBS!

    정기배송으로 진행시 가격도 50만원으로 저렴하고 정해진 날짜에 와서 좋아요!
    DMSO같이 많이 쓰는 제품도 media와 같이 묶어서 사용하면 더 저렴해지더라구요 ㅎ

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