Key Documents

View All Documentation

Fumarase from porcine heart

Name: Fumarase from porcine heart
Brand: SIGMA

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

Synonym(s):

L-Malate hydrolyase, Fumarate hydratase

Select a Size

Change View

Size/SKU	Availability	Price

About This Item

CAS Number:

9032-88-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-887-7

MDL number:

MFCD00131111

EC Number:

4.2.1.2 (BRENDA, IUBMB)

Specific activity:

≥300 units/mg protein (biuret)

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

form

ammonium sulfate suspension

Quality Segment

200

specific activity

≥300 units/mg protein (biuret)

foreign activity

malic dehydrogenase ≤0.1%

storage temp.

2-8°C

Description

General description

Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.

Application

Fumarase from porcine heart has been used:

as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
for immobilized enzyme based stability assay
in fumarate hydratase assays in human lung carcinoma cells (A549)

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.

Biochem/physiol Actions

Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, 0.05 M KH₂PO₄, pH 7.5, 0.014 M 2-mercaptoethanol

Preparation Note

Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.

Other Notes

One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	M7383	M1567	M2634
Sigma-Aldrich F1757 Fumarase from porcine heart	Sigma-Aldrich M7383 Malic Dehydrogenase from porcine heart	Sigma-Aldrich M1567 Malic Dehydrogenase from porcine heart	Sigma-Aldrich M2634 Malic Dehydrogenase from porcine heart
description ammonium sulfate suspension, ≥300 units/mg protein (biuret)	description ≥400 units/mg protein (biuret), ammonium sulfate suspension	description ≥600 units/mg protein (biuret), ammonium sulfate suspension	description buffered aqueous glycerol solution, ≥600 units/mg protein (biuret)
specific activity ≥300 units/mg protein (biuret)	specific activity ≥400 units/mg protein (biuret)	specific activity ≥600 units/mg protein (biuret)	specific activity 600-1000 units/mg protein (biuret)
form ammonium sulfate suspension	form ammonium sulfate suspension	form ammonium sulfate suspension	form buffered aqueous glycerol solution
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
foreign activity malic dehydrogenase ≤0.1%	foreign activity Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%	foreign activity Glutamic-Pyruvic Transaminase ≤0.01%, Glutamic-Oxalacetic Transaminase ≤0.01%	foreign activity Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%
Quality Level 200	Quality Level -	Quality Level 200	Quality Level 200

Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H317

Precautionary Statements

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.