F0522

Monoclonal Anti-CD3−FITC antibody produced in mouse

clone UCHT-1, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-CD3
• NACRES: NA.44
• UNSPSC Code: 12352203
• Conjugate: FITC conjugate
• Clone: UCHT-1, monoclonal
• Application: FACS
• Citations: 5

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: UCHT-1, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: 10 μL using 1 × 10⁶ cells
• isotype: IgG1
• UniProt accession no.: P07766
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD3E(916)

Description

General description

CD3e molecule, ε (CD3E) is a polypeptide encoded by the CD3E gene in humans. It resides on chromosome 11. The CD3ε cytoplasmic domain of the T cell receptor binds to the inner leaflet of the plasma membrane (PM) and is accessible during early stages of T cell activation. It is specific for T lymphocytes and is found in Purkinje cells of the cerebellum as well as in gastric parietal cells of humans and other species. Cytoplasmic cyCD3 expression can only be seen during the early phase of NK-cell differentiation.

The CD3 complex (16/20/29/22/25-28 kDa) consists of 5 polypeptide chains associated with each other and with the T cell receptor.

Immunogen

human thymocytes followed by Sezary T cells.

Application

Monoclonal Anti-CD3-FITC antibody produced in mouse is suitable for flow cytometry (at a dilution of 10μL using 1 × 106 cells). It was also used for ADCC Assay.

Biochem/physiol Actions

Recognizes the CD3 complex. The epitope recognized by clone no. UCHT-1 is expressed on the ε-chain of the CD3 antigen/T cell receptor complex. 3rd Workshop: code no. 126, 208 and 471
4th Workshop: paper no. T3.2

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Preparation Note

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable