EPI001
Histone Acetyltransferase (HAT) Activity Assay Kit
100 assays in 96 well plates
Synonym(s):
HAT Activity, Histone Acetyltransferase Activity
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usage
100 assays in 96 well plates
shipped in
wet ice
storage temp.
−20°C
General description
Histone acetyltransferases (HATs) have critical roles in cellular functions and are involved in the regulation of gene transcription, differentiation, and proliferation. The HAT Activity Colorimetric Assay Kit offers a convenient, non-radioactive system for a rapid and sensitive detection of HAT activity in mammalian samples. The kit includes an active Nuclear Extract (NE) to be used as a positive control, HAT cofactor, acetyl-CoA, and all downstream cofactors and substrates. Acetylation of a peptide substrate by active HAT releases the free form of CoA, which then serves as an essential coenzyme for producing NADH. NADH can easily be detected spectrophotometrically upon reacting with a soluble tetrazolium dye. The detection can be continuous, and therefore, is suitable for kinetic studies. The kit provides all reagents and a simple, straightforward protocol for a complete assay.
Features and Benefits
- Sensitive and reliable assay
- Utilizes colorimetric methods
- Sample type: cell and tissue lysates
- Species reactivity: mammalian
- Suitable for individual tests or high throughput assays and kinetic studies
- Easy and simple procedure: combine nuclear extract or protein sample with reagents, incubate, and read absorbance
- Convenient 96-well microplate format
- Continuously measures HAT activity; suitable for kinetic studies
- No HDAC interference; crude nuclear extract can be used directly
Storage Class Code
12 - Non Combustible Liquids
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