D7679
3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System
liquid
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About This Item
CAS Number:
NACRES:
NA.75
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
231-018-9
MDL number:
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form
liquid
Quality Level
technique(s)
immunoblotting: suitable
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
SMILES string
Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2
InChI
1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
InChI key
KJDSORYAHBAGPP-UHFFFAOYSA-N
1 of 4
This Item | |||
|---|---|---|---|
| solubility water: soluble | solubility - | solubility - | solubility H2O: 50 mg/mL |
| technique(s) immunoblotting: suitable | technique(s) - | technique(s) - | technique(s) titration: 96-104% (with NaOH) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form powder, crystals or chunks |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
General description
DAB (3,3′-Diaminobenzidine Tetrahydrochloride) Liquid Substrate Dropper System has been developed for use in immunohistology and immunoblotting procedures as a precipitating substrate for the detection of peroxidase activity. DAB is the immunohistology substrate of choice because it produces an intense brown stain that is easily observed. The end product is resistant to alcohol, therefore, a variety of counterstains and mounting media can be used with the DAB Liquid Substrate Dropper System. DAB Liquid Substrate Dropper System provides all of the chromogen and buffer/peroxide solutions needed to produce a fast and convenient DAB substrate solution.
Application
3,3′-Diaminobenzidine (DAB) Liquid Substrate Dropper System has been used as a developer in immunohistogical examination.
Preparation Note
Each DAB liquid substrate dropper system will provide ten individual application dropper bottles of 10 mL of a fast and convenient DAB substrate solution.
Disclaimer
Stable at least one year at 2-8 °C.
Kit Components Only
Product No.
Description
- 10× DAB Liquid Chromogen 10 mL
- premeasured, ready-to-use buffer/peroxide solution for individual application 10 x 9
wgk
WGK 3
Storage Class
3 - Flammable liquids
signalword
Danger
hcodes
Hazard Classifications
Flam. Liq. 2
flash_point_f
51.8 °F - closed cup
flash_point_c
11 °C - closed cup
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N M Chu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 37(2), 257-263 (1989-02-01)
Immunoblotting techniques are widely used for detection of antigen immobilized on nitrocellulose membranes. There are many immunolabeling methods and staining methods available to disclose the presence of antigen in such techniques. Five common staining methods each for alkaline phosphatase and
S M Hsu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 30(10), 1079-1082 (1982-10-01)
Three metallic ions, NiCl2, CoCl2, and CuSO4, were found to modify the color of the normally brown diaminobenzidine (DAB) reaction. The colors ranged from purplish blue (NiCl2), dark blue/bluish black (CoCl2), to greyish blue (CuSO4). We have found that the
Cloning and characterization of porcine aquaporin 1 water channel expressed extensively in gastrointestinal system
Jin SY, et al.
World Journal of Gastroenterology, 12(7), 1092-1092 (2006)
The duality of beta-catenin function: a requirement in lens morphogenesis and signaling suppression of lens fate in periocular ectoderm
Smith AN, et al.
Developmental Biology, 285(2), 477-489 (2005)
Carola W N Damen et al.
Analytical biochemistry, 393(1), 73-79 (2009-06-16)
For the bioanalysis of therapeutic monoclonal antibodies in biological matrices, immunoassays--especially enzyme-linked immunosorbent assays (ELISAs)--are the most widely used techniques. Although ELISAs are very sensitive, the obtained sensitivity is not always sufficient. In this study, we have investigated the possibilities
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