D6063

REDTaq^® SuperPak^™ DNA Polymerase

Taq for routine PCR with inert dye; with 10X buffer & dNTP mix

• Synonym(s): DNA polymerase with dNTP, DNA polymerase with loading dye, Taq DNA polymerase
• NACRES: NA.55
• UNSPSC Code: 41106300
• Concentration: 1 unit/μL

Properties

• Quality Segment: 200
• form: liquid
• usage: sufficient for 250 reactions
• feature: dNTPs included, hotstart: no
• concentration: 1 unit/μL
• technique(s): PCR: suitable using : suitable, WGA
• color: red
• input: purified DNA
• suitability: suitable for PCR
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

REDTaq^® SuperPak^™ DNA Polymerase is Sigma′s Taq DNA Polymerase mixed with an inert red dye. REDTaq^® SuperPak^™ DNA Polymerase is a convenient package, that includes all the necessary components for a PCR reaction except primers, DNA template and water. It consists of Sigma′s high quality REDTaq DNA polymerase, 10 mM ultrapure deoxynucleotide mix and 10´ PCR reaction buffer. The dye provides quick recognition of reactions to which enzyme has been added as well as visual confirmation of complete mixing.

Application

REDTaq^® SuperPak^™ DNA Polymerase has been used in:

• reverse transcriptase-polymerase chain reaction (RT-PCR)[1]
• nested PCR[2]
• routine PCR amplification[3]

Features and Benefits

• Contains inert dye that enables visual confirmation of enzyme addition and direct loading onto agarose gel without the need for an additional loading dye
• Suitable for nested PCR
• Contains ultrapure 10mM dNTP mix in a separate vial

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

SuperPak is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

• P2192

  10X PCR Buffer, Optimized for routine PCR with MgCl₂ included

  SDS
• D7295

  Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

  SDS

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: No data available
• flash_point_c: No data available
• wgk: WGK 2