PI.1 Ut

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: raccoon uterus
• packaging: tube of 5 μg 90111909-DNA-5UG; pkg of vial of cells 90111909-1VL
• growth mode: Adherent
• karyotype: 2n = 38
• morphology: Fibroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Racoon uterus

Cell Line Description

Derived from trypsinised uterine tissue of an adult female Racoon. Pl.1Ut was developed for studies of feline and canine viruses since the Racoon is one of the few animals which is susceptible to both.

Application

Virus studies: feline and canine viruses

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² by washing 3 times with 0.255 trypsin or trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan; maximum 90 passages expected. Growth rate is very slow.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.