m17.ASC

11112201, mouse adipose tissue, Fibroblast-like

• Synonym(s): m17 ASC, m17ASC
• UNSPSC Code: 41106514

Properties

• product name: m17.ASC, 11112201
• biological source: mouse adipose tissue
• growth mode: Adherent
• karyotype: Normal diploid
• morphology: Fibroblast-like
• receptors: Sca-1, c-kit/CD117, nestin, nucleostemin, CD44 and CD106

Description

Cell Line Origin

Adipose cell line, mouse subcutaneous, mesenchymal, multipotent

Cell Line Description

m17.ASC is a spontaneously immortalized multipotent mesenchymal cell line capable of acquiring adipocyte, chondrocyte, osteocyte and cardiomyocyte phenotypes when cultured with appropriate differentiation factors. m17.ASC provides a valuable tool for in vitro biomimetic studies; assessing growth technologies such as 3-D structures, biochemical cues (selected growth factors and hormones) and physical forces (electrical, mechanical and topographical). It can be used to screen compounds that induce or inhibit proliferation and/or differentiation in tissue specific phenotypes.

DNA Profile

Not specified

Culture Medium

51800C (51800C) + L-Glutamine (G7513) + FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (90%) approximately 1:6 with media changes or subculture every 2-3 days seeding at 0.5-4 x 10⁴ cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

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