C8743
Cholesterol Dehydrogenase from Nocardia sp.
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About This Item
Specific activity:
≥18 unit/mg solid
Recombinant:
expressed in Streptomyces lividans
Pricing and availability is not currently available.
recombinant
expressed in Streptomyces lividans
Quality Segment
form
lyophilized solid
specific activity
≥18 unit/mg solid
mol wt
~37 kDa by SDS-PAGE
storage temp.
−20°C
Application
Enzyme used in determining cholesterol in serum
The enzyme sequence is derived from Nocardia sp., and this cholesterol dehydrogenase is expressed in Streptomyces lividans. Cholesterol Dehydrogenase from Nocardia sp. has been used in the preparation of amperometric cholesterol biosensor for cholesterol detection in biological samples and in a study to investigate the atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain with the LDL-receptor tail. Cholesterol dehydrogenase from Nocardia sp. Has also been used in a study to assess organization, dynamics, and segregation of Ras nanoclusters in membrane domains.
Biochem/physiol Actions
Cholesterol dehydrogenase is a NAD(P) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Molecular mass of the protein is found to be 37 kDa based on SDS-PAGE. Its pH is 4.5.
Cholesterol dehydrogenase (CDH) is a nicotinamide adenine dinucleotide phosphate (NAD(P)) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Cholesterol detection by using CDH allows direct and easier quantification compared to cholesterol oxidase based assay.
Physical form
Supplied as a lyophilized powder
Other Notes
One unit produces 1.0 μmole of cholesten-4-en-3-one per minute at pH 8.5 at 25 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥18 unit/mg solid | specific activity ≥200 units/g protein | specific activity 8-20 units/mg solid | specific activity ≥45 units/mg solid |
| recombinant expressed in Streptomyces lividans | recombinant - | recombinant - | recombinant expressed in E. coli |
| form lyophilized solid | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~37 kDa by SDS-PAGE | mol wt 84 kDa by SDS-PAGE | mol wt 71 kDa by SDS-PAGE, 83 kDa by gel filtration | mol wt 56 kDa by SDS-PAGE |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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