This product, Chymase human, is an active form with activity specifications of ≥40 units/mg protein, indicating it is a mature form. The sequence information is proprietary and not available.
C8118
Chymase human
recombinant, expressed in Pichia pastoris
Synonym(s):
Mast cell protease I
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥40 units/mg protein
Recombinant:
expressed in Pichia pastoris
Concentration:
125-400 μg/mL
Pricing and availability is not currently available.
recombinant
expressed in Pichia pastoris
Quality Segment
form
liquid
specific activity
≥40 units/mg protein
mol wt
~37 kDa by SDS-PAGE
concentration
125-400 μg/mL
UniProt accession no.
application(s)
diagnostic assay manufacturing
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CMA1(1215)
General description
Chymase is a cathepsin G-like, S1 serine proteinase found primarily in mast cells. It has a molecular mass of ~30 kDa, however its apparent molecular mass on SDS-PAGE is around 37 kDa.
Application
Chymase has been implicated in generation of angiotensin II and cleavage of big endothelin. Studies indicate it may be involved in vascular proliferation, myocardial infarction and dermatitis.
Human chymase has been used in a study to assess the effects of Panax notoginseng flower extract on the TGF-β/Smad signal transduction pathway in heart remodeling. Human chymase has also been used in a study to investigate the blood glucose level and survival in streptozotocin-treated human chymase transgenic mice.
Biochem/physiol Actions
Physical form
Supplied as a solution in 20 mM Tris, 0.8 M NaCl and 25% glycerol, pH 7.6
Other Notes
One unit hydrolyzes one micromole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7.8 and 25 °C. The assay buffer used to determine the enzyme activity contains 27 mM Tris-HCl, pH 7.8, with 150 mM NaCl and 0.43 mM BTEE.
1 of 1
This Item | |||
|---|---|---|---|
| description recombinant, expressed in Pichia pastoris | description clone B7, 2.4 mg/mL, Chemicon® | description Type II, lyophilized powder, ≥40 units/mg protein | description ≥93% (SDS-PAGE), recombinant, expressed in E. coli |
| Gene Information human ... CMA1(1215) | Gene Information human ... CMA1(1215) | Gene Information cow ... CTRB1(618826) | Gene Information Pyrococcus furiosus DSM 3638 ... PF0541(1468383) |
| specific activity ≥40 units/mg protein | specific activity - | specific activity ≥40 units/mg protein | specific activity 0.5 units/mg protein |
| form liquid | form - | form lyophilized powder | form solution |
| application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing | application(s) - |
| recombinant expressed in Pichia pastoris | recombinant - | recombinant - | recombinant expressed in E. coli |
| concentration 125-400 μg/mL | concentration 2.4 mg/mL | concentration - | concentration - |
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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CMA1 has a pro form starting at amino acid 20 and a mature form starting at amino acid 22. Can you tell me which form this is or the N-term origin?
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