C7688
Chaperonin 60 from Escherichia coli
>95% (SDS-PAGE), recombinant, expressed in E. coli overproducing strain, lyophilized powder
Synonym(s):
Cpn60, GroEL
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biological source
Escherichia coli
Quality Level
recombinant
expressed in E. coli overproducing strain
Assay
>95% (SDS-PAGE)
form
lyophilized powder
technique(s)
electron microscopy: suitable
mass spectrometry (MS): suitable
suitability
passes test (Refolding (w/GroES))
UniProt accession no.
storage temp.
2-8°C
Gene Information
human ... HSPD1(3329)
General description
Research area: Cell Signaling
Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles.
Chaperonin 60 (GroEL) and chaperonin 10 (GroES) belong to the ubiquitous family of heat-shock molecular chaperones found in prokaryotes and in eukaryotic organelles.
Application
Chaperonin 60 from Escherichia coli has been used:
- in mass spectroscopy[1]
- in cryo-electron microscopy imaging as control for testing particle distribution[2]
- as standard in infrared spectrum measurements[3]
- as a protein sample for stability assessment and characterization studies using differential mobility analysis (DMA) and cryo-electron microscopy (cryo-EM).[4]
Biochem/physiol Actions
Apart from its role in facilitating protein folding, chaperonin 60 (Cpn60) serves as an extracellular signaling protein, showing functional similarities to certain proinflammatory cytokines.[5] Furthermore, Cpn60 contributes to the inhibition of lipid accumulation and adipogenesis during the initial stages of cellular differentiation, thereby exerting anti-obesity effects.[6]
Packaging
Package size based on protein content.
Physical form
Lyophilized powder containing Tris buffer salts, potassium chloride, magnesium chloride, dithiothreitol, and trehalose as stabilizer.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Infrared irradiation in the collision cell of a hybrid tandem quadrupole/time-of-flight mass spectrometer for declustering and cleaning of nanoelectrosprayed protein complex ions
El-Faramawy A, et al.
Analytical Chemistry, 82(23), 9878-9884 (2010)
Observation of a single protein by ultrafast X-ray diffraction
Ekeberg T, et al.
Light: Science & Applications, 13(1), 15-15 (2024)
Chaperonin 60 unfolds its secrets of cellular communication
Maguire M, et al.
Cell Stress & Chaperones, 7(4), 317-329 (2002)
Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports
Meyerson JR, et al.
Scientific Reports, 4(2), 7084-7084 (2014)
Surface-exposed chaperonin 60 derived from Propionibacterium freudenreichii MJ2 inhibits adipogenesis by decreasing the expression of C/EBP?/PPAR?
An M and Lim Y-H
Scientific Reports, 13(1), 19251-19251 (2023)
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