C7089
Monoclonal Anti-Cdk7/CAK antibody produced in mouse
clone MO-1.1, ascites fluid
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
MO-1.1, monoclonal
mol wt
antigen 40 kDa
contains
15 mM sodium azide
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:50,000 using a cultured human cell line extract
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDK7(1022)
General description
Cell division protein kinase 7 is an enzymes encoded by the CDK7 gene in humans. The protein belongs to cyclin-dependent protein kinase (CDK) family. CAK is a CDK-cyclin complex implicated in the control of multiple cell cycle transitions. The structure of CDK7 facilitates the inhibitors with differential specificity for the different CDKs and can be used in different biological processes.
Specificity
The antibody reacts specifically with Cdk7 (40 kDa catalytic subunit of Cdk activating kinase).
Immunogen
recombinant human Cdk7 protein.
Application
Monoclonal Anti-Cdk7/CAK antibody produced in mouse is suitable for the following applications:
- Immunocytochemistry
- Immunohistochemistry (frozen sections)
- Immunoprecipitation
- Microarray
- Western blotting at a dilution of 1:50,000 using a cultured human cell line extract
Biochem/physiol Actions
Cyclin-dependent kinase (CDK) Activating Kinase (CAK) is involved in activation and phosphorylation of CDK1, CDK2, CDK4 and CDK6 and regulation of the cell cycle. It is required for cell cycle progression and it could be a target for cancer therapy. CAK plays a central role in regulation of messenger RNA synthesis by either inhibition of RNA polymerase II-catalyzed transcription or stimulation of transcription through association with basal transcription repair factor IIH. Phosphorylation by the CDK-activating kinase (CAK) is required for the activation of cyclin-dependent kinases.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Stéphane Larochelle et al.
Methods in molecular biology (Clifton, N.J.), 296, 279-290 (2004-12-04)
All cyclin-dependent kinases (CDKs) involved in eukaryotic cell cycle control require phosphorylation at a conserved threonine (or serine) residue within the activation- or T-loop to attain full enzymatic activity. The enzyme responsible for this activating phosphorylation, the CDK-activating kinase (CAK)
Graziano Lolli et al.
Cell cycle (Georgetown, Tex.), 4(4), 572-577 (2005-05-07)
The Cyclin-dependent kinase (CDK) Activating Kinase (CAK) is responsible for the activating phosphorylation of CDK1, CDK2, CDK4 and CDK6 and regulation of the cell cycle. The kinase is composed of three subunits: CDK7, Cyclin H and MAT1 (ménage a trois).
Joel Rovnak et al.
Journal of virology, 86(10), 5742-5751 (2012-03-02)
Alterations in the functional levels of cyclin-dependent kinase-8 (CDK8) or its partner, cyclin C, have been clearly associated with cancers, including colon cancer, melanoma, and osteosarcoma. Walleye dermal sarcoma virus encodes a retroviral cyclin (RV-cyclin) that localizes to interchromatin granule
D A Bochar et al.
The Journal of biological chemistry, 274(19), 13162-13166 (1999-05-01)
Cyclin-dependent kinase 7 (CDK7) can be isolated as a subunit of a trimeric kinase complex functional in activation of the mitotic promoting factor. In this study, we demonstrate that the trimeric cdk-activating kinase (CAK) acts as a transcriptional repressor of
R P Fisher et al.
Cell, 78(4), 713-724 (1994-08-26)
Phosphorylation by the CDK-activating kinase (CAK) is a required step in the activation of cyclin-dependent kinases. We have purified CAK from mammalian cells; the enzyme comprises two major polypeptides of 42 and 37 kDa. Protein sequencing indicates that the 42
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