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Cathepsin B from bovine spleen

Name: Cathepsin B from bovine spleen
Brand: SIGMA

lyophilized powder, ≥10 units/mg protein

Synonym(s):

Cathepsin B₁

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About This Item

CAS Number:

9047-22-7

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

3.4.22.1 (BRENDA, IUBMB)

MDL number:

MFCD02662345

Specific activity:

≥10 units/mg protein

Biological source:

bovine spleen

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Properties

biological source

bovine spleen

Quality Segment

200

form

lyophilized powder

specific activity

≥10 units/mg protein

composition

Protein, ≥30% biuret

UniProt accession no.

P07688

storage temp.

−20°C

Gene Information

cow ... CTSB(281105)

Description

General description

Cathepsin B is a lysosomal enzyme that comprises a light chain (Lys1–Arg49) and a heavy chain (Val50–Thr253). It belongs to cysteine family C1.

Application

Cathepsin B from bovine spleen has been used in cleavage assay before high-performance liquid chromatography-mass spectrometry (HPLC-MS) analysis. It has also been used in the enzyme inhibition assays with protease inhibitors RfIP1 and ruthenium metalloarenes.

Biochem/physiol Actions

Cathepsin B displays an endopeptidase and peptidyl dipeptidase activities. It may be associated with the pathophysiology of tumors. Cathepsin B is also regarded as a prominent protease in Leishmaniasis. It is overactivated in muscular dystrophy, pulmonary emphysema, and bone resorption.

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.

Physical form

Lyophilized powder containing sodium phosphate, sodium chloride and ~6% EDTA as stabilizer.

Other Notes

One unit will hydrolyze 1 μmole of Z-lysine p-nitrophenyl ester per min at pH 5.0 at 25 °C.

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This Item	C0150	C8696	SRP0289
Sigma-Aldrich C6286 Cathepsin B from bovine spleen	Sigma-Aldrich C0150 Cathepsin B from human placenta	Sigma-Aldrich C8696 Cathepsin D from human liver	Sigma-Aldrich SRP0289 Cathepsin B Active human
description lyophilized powder, ≥10 units/mg protein	description lyophilized powder, ≥5 units/mg protein	description lyophilized powder, ≥250 units/mg protein (E1%/280)	description recombinant, expressed in FreeStyle^™ 293-F cells, ≥90% (SDS-PAGE)
biological source bovine spleen	biological source -	biological source -	biological source human
Gene Information cow ... CTSB(281105)	Gene Information human ... CTSB(1508)	Gene Information human ... CTSD(1509)	Gene Information human ... CTSB(1508)
specific activity ≥10 units/mg protein	specific activity ≥5 units/mg protein	specific activity ≥250 units/mg protein (E1%/280)	specific activity ≥2228 pmol/min-μg
form lyophilized powder	form lyophilized powder	form lyophilized powder	form aqueous solution
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −70°C
UniProt accession no. P07688	UniProt accession no. P07858	UniProt accession no. P07339	UniProt accession no. -

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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