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Cyanoborohydride Coupling Buffer

Name: Cyanoborohydride Coupling Buffer
Brand: SIGMA

Synonym(s):

Coupling buffer

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About This Item

NACRES:

NA.56

UNSPSC Code:

12161700

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Properties

form

liquid

Quality Segment

200

reaction suitability

reagent type: reductant

technique(s)

affinity chromatography: suitable

application(s)

life science and biopharma

storage temp.

2-8°C

Description

Application

A ready-to-use reagent used to couple amine ligands to aldehyde functional groups. The coupling buffer reaction is a reductive amination of the intermediate Schiff′s base to a stable C−N bond.

Cyanoborohydride Coupling Buffer is used in affinity chromatography, protein chromatography, activated/functionalized matrices and synthetic reagents. Cyanoborohydride has been used to inform a safe and effective gene-transfer system targeting hepatocytes as well as to develop a method for targeted delivery of anticancer therapeutics to cancer cells in hypoxic areas.

Cyanoborohydride Coupling Buffer has been used:

in coupling reactions between amines and glutaraldehyde
to reduce hydrazone bond to a stable hydrazide bond
as a component in oligonucleotide reaction mixture for coverslips functionalization

Biochem/physiol Actions

Cyanoborohydride Coupling Buffer is a reagent suitable for reductive amination processes, that contributes to transformation of simple alcohols into more complex amines. It is used in the conversion of Schiff base, by reducing it, to form a secondary amine without affecting aldehyde groups on the support.

Preparation Note

0.02 M sodium phosphate, pH 7.5, containing 0.2 M sodium chloride and 3.0 g/L sodium cyanoborohydride

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This Item	674931	526304	85810
Sigma-Aldrich C4187 Cyanoborohydride Coupling Buffer	Sigma-Aldrich 674931 Cyanoborohydride, silica-supported	Sigma-Aldrich 526304 Cyanoborohydride, polymer supported	Sigma-Aldrich 85810 STE buffer solution
description -	description -	description macroporous, 20-50 mesh, extent of labeling: ~2.0-3.5 mmol/g loading	description BioUltra, Molecular Biology, pH 7.8
technique(s) affinity chromatography: suitable	technique(s) -	technique(s) -	technique(s) -
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 200
form liquid	form -	form macroporous	form liquid
application(s) life science and biopharma	application(s) -	application(s) -	application(s) -
storage temp. 2-8°C	storage temp. -	storage temp. 2-8°C	storage temp. -
reaction suitability reagent type: reductant	reaction suitability reactivity: imine reactive, reaction type: solution phase peptide synthesis	reaction suitability reaction type: solution phase peptide synthesis, reactivity: imine reactive	reaction suitability -

Pictograms

GHS07,GHS09

Signal Word

Warning

Hazard Codes

H302 + H312,H411

Precautionary Statements

P264 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P362 + P364

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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