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Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

Name: Choloylglycine Hydrolase from <I>Clostridium perfringens (C. welchii)</I>
Brand: SIGMA

lyophilized powder, ≥100 units/mg protein

Synonym(s):

3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase

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About This Item

CAS Number:

37289-07-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.5.1.24 (BRENDA, IUBMB)

MDL number:

MFCD00130789

Specific activity:

≥100 units/mg protein

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Properties

form

lyophilized powder

Quality Segment

200

specific activity

≥100 units/mg protein

composition

Protein, ≥50% biuret

shipped in

wet ice

storage temp.

−20°C

Description

Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg²⁺, Cu²⁺, and Cd²⁺) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Other Notes

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

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This Item	N2876	N2133	P7633
Sigma-Aldrich C4018 Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)	Sigma-Aldrich N2876 Neuraminidase from Clostridium perfringens (C. welchii)	Sigma-Aldrich N2133 Neuraminidase from Clostridium perfringens (C. welchii)	Sigma-Aldrich P7633 Phospholipase C from Clostridium perfringens (C. welchii)
description lyophilized powder, ≥100 units/mg protein	description Suitable for manufacturing of diagnostic kits and reagents, Type V, lyophilized powder	description Type X, lyophilized powder, ≥50 units/mg protein (using 4MU-NANA)	description Type I, lyophilized powder, 10-50 units/mg protein
specific activity ≥100 units/mg protein	specific activity ≥1.3 units/mg solid (using 4MU-NANA), ≥0.1 units/mg solid (using mucin)	specific activity ≥50 units/mg protein (using 4MU-NANA)	specific activity 10-50 units/mg protein
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
shipped in wet ice	shipped in dry ice	shipped in -	shipped in -
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200
composition Protein, ≥50% biuret	composition -	composition -	composition protein, 20-40%

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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