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C0888

Clostripain from Clostridium histolyticum

≥20 units/mg solid

Synonym(s):

Clostridiopeptidase B, Proteinase from Clostridium histolyticum

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About This Item

CAS Number:
9028-00-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-822-2
MDL number:
MFCD00130814
EC Number:
3.4.22.8 (BRENDA, IUBMB)
Specific activity:
≥20 units/mg solid
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form

lyophilized powder

Quality Segment

specific activity

≥20 units/mg solid

mol wt

15.4 kDa, 41.7 kDa

impurities

salt, essentially free

storage temp.

2-8°C

Application

Clostripain from Clostridium histolyticum has been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit. It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43).

Biochem/physiol Actions

Clostripain from Clostridium histolyticum is composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Analysis Note

The enzyme must be activated for 2-3 hours before use by dissolving in 2.5 mM dithiothreitol containing 1.0 mM calcium acetate.
Purified, essentially salt-free

Other Notes

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of 2.5 mM DTT.

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This Item
C5894P3910P4032
Clostripain from Clostridium histolyticum ≥20 units/mg solid

Sigma-Aldrich

C0888

Clostripain from Clostridium histolyticum

Collagenase from Clostridium histolyticum non-sterile; 0.2 μm filtered, Type IA-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Sigma-Aldrich

C5894

Collagenase from Clostridium histolyticum

Protease from Bacillus licheniformis lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

Sigma-Aldrich

P3910

Protease from Bacillus licheniformis

Proteinase from Aspergillus melleus Type XXIII, ≥3 units/mg solid

Sigma-Aldrich

P4032

Proteinase from Aspergillus melleus

description

≥20 units/mg solid

description

non-sterile; 0.2 μm filtered, Type IA-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

description

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

description

Type XXIII, ≥3 units/mg solid

specific activity

≥20 units/mg solid

specific activity

≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid

specific activity

-

specific activity

≥3 units/mg solid

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

solid

mol wt

41.7 kDa, 15.4 kDa

mol wt

68-130 kDa

mol wt

27 kDa

mol wt

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

impurities

salt, essentially free

impurities

-

impurities

-

impurities

-

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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