B8438

Alcian Blue solution

1% in 3% acetic acid, pH 2.5

• Synonym(s): Alcian Blue 8XG solution
• NACRES: NA.47
• UNSPSC Code: 12171500

Properties

• form: liquid
• Quality Segment: 100
• concentration: 1% in 3% acetic acid, pH 2.5
• technique(s): microbe id | staining: suitable
• color: dark blue to very dark blue
• pH range: 2.4-2.6
• Amax: 0.45-0.60 (1:400 in H2O)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp

Description

Application

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

Biochem/physiol Actions

Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable