AB0300
Alkaline Phosphatase Blue Membrane Substrate Solution
alkaline phosphatase substrate, solution
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.83
eCl@ss:
42010105
Pricing and availability is not currently available.
Product Name
Alkaline Phosphatase Blue Membrane Substrate Solution, sufficient for 400 mL working substrate
form
solution
Quality Segment
usage
sufficient for 400 mL working substrate
technique(s)
protein detection: suitable
storage temp.
2-8°C
General description
Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors.
Application
Alkaline Phosphatase Blue Membrane Substrate Solution has been used:
- to analyse direct alkaline phosphatase (AP) activity
- to perform alkaline phosphatase assays with colorimetric system
- to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3
Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays.
Physical form
A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)
Legal Information
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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