A9378

L-Amino Acid Oxidase from Crotalus adamanteus

Type IV, ≥4.0 units/mg protein, aqueous suspension

• Synonym(s): L-AAO, L-Amino acid:oxygen oxidoreductase (deaminating)
• CAS Number: 9000-89-9
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-564-0
• MDL number: MFCD00131454
• EC Number: 1.4.3.2 (BRENDA, IUBMB)
• Specific activity: ≥4.0 units/mg protein
• Concentration: ≥5.0 mg/mL

Properties

• type: Type IV
• Quality Segment: 200
• form: aqueous suspension
• specific activity: ≥4.0 units/mg protein
• mol wt: ~130 kDa
• contains: toluene as preservative
• concentration: ≥5.0 mg/mL
• solubility: H₂O: soluble 1.0 mg/mL, clear
• storage temp.: 2-8°C

Description

Application

L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. ³⁵S dimethylsulfoniopropionate (DMSP) has been synthesized chemically from ³⁵S L-methionine using LAAO from Sigma to form ³⁵S 3-methiolpropionate.

Biochem/physiol Actions

L-Amino acid oxidase is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70,000 Da. Each molecule of holoenzyme has two FAD molecules. It is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. Optimum pH is approximately 7.5.The enzyme may be reversibly inactivated by incubation in phosphate buffer, pH 7.5 at 38 °C. L-amino acid oxidase is involved in various metabolic pathways such as alanine and aspartate metabolism, methionine metabolism, valine, leucine and isoleucine degradation, tyrosine metabolism, phenylalanine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alkaloid biosynthesis. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney and liver.

Packaging

Package size based on protein content

Preparation Note

Dissolves in water at 1 mg/mL concentration to form a clear solution.

Other Notes

One Unit oxidizes one micromole of L-leucine per minute at 25 °C, pH 7.6

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Codes: H300 + H310 + H330
• Precautionary Statements: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)