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A8706

Sigma-Aldrich

Avidin from egg white

recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)

Synonym(s):

AVID_CHICK

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About This Item

CAS Number:
1405-69-2
EC Number:
215-783-6
MDL number:
MFCD00130572
UNSPSC Code:
12352202
NACRES:
NA.56

recombinant

expressed in corn

Assay

≥80% protein basis (biuret)

form

powder

specific activity

≥12 units/mg protein (E1%/280)

mol wt

glycoprotein 66 kDa
subunit 16 kDa

technique(s)

ELISA: suitable
immunoblotting: suitable
immunoelectrophoresis: suitable

UniProt accession no.

P02701

storage temp.

2-8°C

Gene Information

chicken ... AVD(396260)

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General description

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.

Unit Definition

One unit will bind 1.0 μg of d-biotin.

Preparation Note

Affinity purified

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Commercial Plant-Produced Recombinant Protein Products, 15-25 (2014)
Sofia H L Frost et al.
Cancer biotherapy & radiopharmaceuticals, 28(2), 108-114 (2012-12-13)
Abstract Purpose: Pretargeted radioimmunotherapy (PRIT) against intraperitoneal (i.p.) ovarian microtumors using avidin-conjugated monoclonal antibody MX35 (avidin-MX35) and (211)At-labeled, biotinylated, succinylated poly-l-lysine ((211)At-B-PLsuc) was compared with conventional radioimmunotherapy (RIT) using (211)At-labeled MX35 in a nude mouse model. Mice were inoculated i.p.
Biopharmaceuticals in Plants: Toward the Next Century of Medicine, 121-122 (2009)
Tom J A Kokhuis et al.
Ultrasound in medicine & biology, 39(3), 490-506 (2013-01-26)
In this study, we investigated the effect of secondary Bjerknes forces on targeted microbubbles using high-speed optical imaging. We observed that targeted microbubbles attached to an underlying surface and subject to secondary Bjerknes forces deform in the direction of their
Oleksii Dubrovskyi et al.
Laboratory investigation; a journal of technical methods and pathology, 93(2), 254-263 (2012-12-06)
Alterations of cell monolayer integrity and increased vascular permeability are key to many pathologies, including atherosclerosis, stroke, lung injury, cancer, digestive disorders and others. Current approaches to probe cell permeability require specific culture conditions and provide an average estimation of
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