A9275

Avidin from egg white

BioUltra, lyophilized powder, ≥10 units/mg protein (E1%/280), ≥98% (SDS-PAGE)

• CAS Number: 1405-69-2
• UNSPSC Code: 12352203
• EC Number: 215-783-6
• NACRES: NA.46
• MDL number: MFCD00130572
• Form: lyophilized powder
• Assay: ≥98% (SDS-PAGE)
• Biological source: egg white
• Mol wt: glycoprotein 66 kDa, subunit 16 kDa

Properties

• biological source: egg white
• Quality Segment: 200
• product line: BioUltra
• assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• specific activity: ≥10 units/mg protein (E1%/280)
• mol wt: glycoprotein 66 kDa, subunit 16 kDa
• storage temp.: 2-8°C

Description

General description

Avidin protein is a homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues[1]. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.

Egg white avidin has been used as a cross-linker for biotin-conjugated antibodies[2]. The product has also been used as a blocking agent in ELISA applications[3].

Preparation Note

Affinity purified

Other Notes

One unit will bind 1.0 μg of d-biotin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)