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N-Acetylneuraminic Acid Aldolase from microorganisms

Name: N-Acetylneuraminic Acid Aldolase from microorganisms
Brand: SIGMA

lyophilized powder, ≥20 units/mg protein (biuret)

Synonym(s):

N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase

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About This Item

CAS Number:

9027-60-5

NACRES:

NA.32

UNSPSC Code:

12352204

Beilstein/REAXYS Number:

2697172

MDL number:

MFCD00131656

EC Number:

4.1.3.3 (BRENDA, IUBMB)

Specific activity:

≥20 units/mg protein (biuret)

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Properties

form

lyophilized powder

Quality Segment

200

specific activity

≥20 units/mg protein (biuret)

mol wt

~98 kDa

storage temp.

−20°C

Description

General description

Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾³M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg⁺⁺, Ag⁺
Optimum pH: 7.5– 8.0
Optimum temp: 70°C
pH Stability: pH 6.0–9.0 (10°C, 25hr)
Thermal stability: Below 65°C (pH 7.5, 30 min)

Application

This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.

Used in the Sialic Acid Quantification Kit, SIALIC-Q

Physical form

Lyophilized powder containing mannitol and EDTA

Other Notes

One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 °C.

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This Item	S1826	110138	A2388
Sigma-Aldrich A6680 N-Acetylneuraminic Acid Aldolase from microorganisms	Sigma-Aldrich S1826 Sialic Acid Aldolase from Escherichia coli K12	Millipore 110138 N-Acetylneuraminic Acid, Synthetic	Sigma-Aldrich A2388 N-Acetylneuraminic acid
description lyophilized powder, ≥20 units/mg protein (biuret)	description recombinant, expressed in E. coli BL21, ≥3.0 units/mg protein	description A component of mucoproteins, mucopolysaccharides, and mucolipids.	description ≥98% (HPLC), from Escherichia coli
specific activity ≥20 units/mg protein (biuret)	specific activity ≥3.0 units/mg protein	specific activity -	specific activity -
form lyophilized powder	form lyophilized powder	form solid	form powder
mol wt ~98 kDa	mol wt 33.4 kDa	mol wt -	mol wt -
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 300

Storage Class

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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