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Azure B

Name: Azure B
Brand: SIGMA

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

N,N,N′-Trimethylthionin, Azure I, Methyleneazure

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About This Item

Empirical Formula (Hill Notation):

C₁₅H₁₆ClN₃S

CAS Number:

531-55-5

Molecular Weight:

305.83

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24890740

EC Number:

208-511-2

MDL number:

MFCD00011935

Colour Index Number:

52010

Beilstein/REAXYS Number:

3922630

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Properties

Product Name

Azure B, certified by the BSC

agency

certified by the BSC

Quality Segment

200

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

dark green

mp

205-210 °C (dec.) (lit.)

solubility

H₂O: 1 mg/mL

fluorescence

λ_ex 648 in ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

C[N+](C)=C(C=C1)C=C(C1=N2)SC3=C2C=CC(NC)=C3.[Cl-]

InChI

1S/C15H15N3S.ClH/c1-16-10-4-6-12-14(8-10)19-15-9-11(18(2)3)5-7-13(15)17-12;/h4-9H,1-3H3;1H

InChI key

DNDJEIWCTMMZBX-UHFFFAOYSA-N

Description

Application

Certified for use in Lillie′s modified Nocht′s method for paraffin sections and the NCCLS method for blood smears.

Azure B has been used for the staining of chromosomes.

Biochem/physiol Actions

Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for the staining of blood films. It is also used for the localization of nucleic acids.

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This Item	227935	1046351	PHR2484
Sigma-Aldrich A4043 Azure B	Sigma-Aldrich 227935 Azure B	USP 1046351 Azure B	Supelco PHR2484 Azure B
description certified by the BSC	description prepared by direct synthesis	description United States Pharmacopeia (USP) Reference Standard	description Pharmaceutical Secondary Standard; Certified Reference Material
technique(s) microbe id \| staining: suitable	technique(s) -	technique(s) -	technique(s) -
color dark green	color -	color -	color -
fluorescence λ_ex 648 in ethanol	fluorescence -	fluorescence -	fluorescence -
application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) pharmaceutical (small molecule)	application(s) pharmaceutical small molecule
solubility H₂O: 1 mg/mL	solubility water: 1 mg/mL, blue to very deep blue	solubility -	solubility -
form powder	form powder	form -	form powder

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Pictograms

GHS07

Signal Word

Warning

Hazard Codes

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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