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A0919

Mouse IgG−Agarose

(Suspension in 0.5 M NaCl containing preservative.)

Synonym(s):

IgG-Agarose Beads

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00164808
Conjugate:
agarose conjugate
Technique(s):
immunoprecipitation (IP): suitable
Application:
IP
Citations:
92
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conjugate

agarose conjugate

Quality Segment

antibody form

purified immunoglobulin

form

(Suspension in 0.5 M NaCl containing preservative.)

extent of labeling

1 mg per mL

technique(s)

immunoprecipitation (IP): suitable

matrix

Cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix spacer

1 atom

storage temp.

2-8°C

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.

Application

IgG-Agarose can be used as an immunoadsorbent. It can also be used to affinity purify antibodies, remove species specific cross-reacting antibodies or remove contaminating antibodies from an antiserum preparation. Typically cross-reacting antibodies may be removed from an antiserum preparation using an equal volume of IgGAgarose (resin volume). Mouse IgG-Agarose has been used for several immunoprecipitation assays,.
Mouse IgGs conjugated to agarose beads were used in immunoprecipitation assays to pre-clear HEK 293 whole cell extracts. Beads were incubated with extracts for 1 hour rotating at 4°C.

Physical form

Suspension in 0.5 M NaCl containing preservative.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A6531A2909A3438
Mouse IgG−Agarose (Suspension in 0.5 M NaCl containing preservative.)

Sigma-Aldrich

A0919

Mouse IgG−Agarose

Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat affinity isolated antibody, suspension

Sigma-Aldrich

A6531

Anti-Mouse IgG (whole molecule)−Agarose antibody produced in goat

Rabbit IgG−Agarose saline suspension

Sigma-Aldrich

A2909

Rabbit IgG−Agarose

Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3438

Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

description

(Suspension in 0.5 M NaCl containing preservative.)

description

affinity isolated antibody, suspension

description

saline suspension

description

affinity isolated antibody, buffered aqueous glycerol solution

conjugate

agarose conjugate

conjugate

agarose conjugate

conjugate

agarose conjugate

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

-

antibody form

affinity isolated antibody

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

(Suspension in 0.5 M NaCl containing preservative.)

form

suspension

form

saline suspension

form

buffered aqueous glycerol solution

technique(s)

immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, Ouchterlony double diffusion: suitable

technique(s)

immunoprecipitation (IP): suitable

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

extent of labeling

1 mg per mL

extent of labeling

≥5 mg per mL

extent of labeling

≥5 mg per mL

extent of labeling

-

Storage Class

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Codes

H317

Hazard Classifications

Skin Sens. 1

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