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Ascorbate Oxidase from Cucurbita sp.

Name: Ascorbate Oxidase from <I>Cucurbita</I> sp.
Brand: SIGMA

lyophilized powder, 1,000-3,000 units/mg protein

Synonym(s):

L-Ascorbate:oxygen oxidoreductase, Ascorbase

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About This Item

CAS Number:

9029-44-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-852-6

MDL number:

MFCD00130560

EC Number:

1.10.3.3 (BRENDA, IUBMB)

Specific activity:

1,000-3,000 units/mg protein

Biological source:

plant (Cucurbita spp.)

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Properties

biological source

plant (Cucurbita spp.)

Quality Segment

200

form

lyophilized powder

specific activity

1,000-3,000 units/mg protein

storage temp.

2-8°C

Description

General description

Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.

Application

Ascorbate Oxidase from Cucurbita sp. has been used:

as a supplement in the culture medium for differentiation into osteoblasts
to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay
in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations

Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis .

Biochem/physiol Actions

Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.

Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.

Physical form

Lyophilized powder containing buffers and sucrose as stabilizer.

Other Notes

One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.

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This Item	189724	Y0000039	A7631
Sigma-Aldrich A0157 Ascorbate Oxidase from Cucurbita sp.	Sigma-Aldrich 189724 Ascorbate Oxidase, Cucurbita sp.	Y0000039 Sodium ascorbate	Sigma-Aldrich A7631 (+)-Sodium L-ascorbate
description lyophilized powder, 1,000-3,000 units/mg protein	description Native ascorbate oxidase from Cucurbita species. Catalyzes the oxidation of ascorbioxidation of ascorbic acid to dehydroascorbic acid. Suitable for eliminating interference of ascorbic acid in clinical assays and for determination of ascorbic acid.	description European Pharmacopoeia (EP) Reference Standard	description crystalline, ≥98%
specific activity 1,000-3,000 units/mg protein	specific activity ≥1500 U/mg, ≥300 units/mg material	specific activity -	specific activity -
biological source plant (Cucurbita spp.)	biological source -	biological source synthetic	biological source synthetic
form lyophilized powder	form lyophilized	form solid	form crystalline
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. -
Quality Level 200	Quality Level 100	Quality Level -	Quality Level 300

Pictograms

GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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