I accidentally bought this thinking it was MTT salt to use in a cell proliferation assay but it is MTT Formazan, the water insoluble end product! Therefore, it is useless for the assay. I suppose you could use it as a control to optimize your buffer for solubilizing the insoluble Formazan. The description for this product is misleading and should clarify that this is not what you need for an MTT assay, and refer the customer to MTT salt. That said, this is a nice purple crystalline powder and insoluble in water consistent with MTT Formazan, if that is what you actually want.
88417
MTT Formazan
MTT reduction end product, powder
Synonym(s):
1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan
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About This Item
Empirical Formula (Hill Notation):
C18H17N5S
CAS Number:
Molecular Weight:
335.43
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
MDL number:
Pricing and availability is not currently available.
Product Name
MTT Formazan, MTT reduction end product
Quality Segment
form
powder
solubility
chloroform: 10 mg/mL, clear, dark violet
SMILES string
Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
InChI
1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
InChI key
GIFKVQOPAJDJLO-SZVLODPRSA-N
Biochem/physiol Actions
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.
Other Notes
Reviews: Tetrazolium salts and formazans
1 of 1
This Item | |||
|---|---|---|---|
| description MTT reduction end product | description powder | description ≥90% (UV) | description MTT Cell Growth Assay is a colorimetric assay that can be used for either proliferation or complement-mediated cytotoxicity assays. |
| solubility chloroform: 10 mg/mL, clear, dark violet | solubility acetone: 1 mg/mL | solubility dioxane: 50 mg/mL, clear | solubility - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| form powder | form powder | form powder | form - |
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Storage Class
11 - Combustible Solids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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