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72068

Sigma-Aldrich

Sodium hydroxide solution

BioUltra, for molecular biology, 10 M in H2O

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About This Item

Linear Formula:
NaOH
CAS Number:
1310-73-2
Molecular Weight:
40.00
MDL number:
MFCD00003548
UNSPSC Code:
12352316
PubChem Substance ID:
57652395
NACRES:
NA.26
Pricing and availability is not currently available.

grade

for molecular biology

Quality Level

200

vapor pressure

3 mmHg ( 37 °C)

product line

BioUltra

form

liquid

concentration

10 M in H2O
9500-10500 mmol/L (titration, acidimetric)

technique(s)

ELISA: suitable
immunofluorescence: suitable
western blot: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

pH

14

bp

105-140 °C

mp

-12 - -10 °C (10 - 50 °F)

solubility

water: soluble

density

1.320-1.340 g/cm3

cation traces

Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤100 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: ≤0.08
λ: 280 nm Amax: ≤0.05

SMILES string

[OH-].[Na+]

suitability

suitable for Western blot
suitable for molecular biology

application(s)

agriculture
life science and biopharma

InChI

1S/Na.H2O/h;1H2/q+1;/p-1

InChI key

HEMHJVSKTPXQMS-UHFFFAOYSA-M

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General description

Sodium hydroxide (NaOH) is an inorganic, white solid compound, which is found as pellets, flakes, and granules. This highly caustic metallic base and alkali can readily absorb moisture and carbon dioxide in the air. NaOH is deliquescent and soluble in ethanol, water, and methanol.[1]

Application

Sodium hydroxide solution has been used:
  • to prepare reagent A (0.1 M NaOH) for the pH inactivation of endogenous L-lactate by L-lactate dehydrogenase (L-LDH)[2]
  • as a component of fresh alkaline melting solution for the denaturation of the antisense strand[3]
  • to prepare Type-I collagen hydrogels for microfluidic device fabrication[4]

Biochem/physiol Actions

Sodium hydroxide (NaOH) is used in various industries, including textiles, drinking water, soaps, and detergents, pulp and paper manufacturing, and as a drain cleaner.[1]

Other Notes

For additional information on our range of Biochemicals, please complete this form.

comparable product

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H290,H314

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM4127
BCCL4493
BCCM0038
BCCL7667
BCCM4416

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Possible materials for producing Geopolymer concrete and its performance with and without Fibre addition-A State of the art review
Kirupa JP Alwis Deva and Sakthieswaran N
International journal of civil and structural engineering, 5(3), 296-296 (2015)
Rikke Wehner Rasmussen et al.
Scandinavian journal of clinical and laboratory investigation, 81(4), 312-317 (2021-04-22)
Intestinal infarction is the fast-evolving endpoint of impaired blood perfusion to an intestinal segment which may have fatal outcome. Early diagnosis and treatment within 6 h reduce mortality. Currently, d-lactate is a promising biomarker, however, not available in the acute clinical
Xin Cui et al.
Biomaterials, 161, 164-178 (2018-02-09)
Glioblastoma (GBM) is the most lethal primary adult brain tumor and its pathology is hallmarked by distorted neovascularization, diffuse tumor-associated macrophage infiltration, and potent immunosuppression. Reconstituting organotypic tumor angiogenesis models with biomimetic cell heterogeneity and interactions, pro-/anti-inflammatory milieu and extracellular
C Berger et al.
Human reproduction (Oxford, England), 30(4), 800-811 (2015-03-06)
Does ulipristal acetate (UPA) used for emergency contraception (EC) interfere with the human embryo implantation process? UPA, at the dosage used for EC, does not affect human embryo implantation process, in vitro. A single pre-ovulatory dose of UPA (30 mg)
Valdeir Arantes et al.
Biotechnology for biofuels, 7, 87-87 (2014-07-01)
There is still considerable debate regarding the actual mechanism by which a "cellulase mixture" deconstructs cellulosic materials, with accessibility to the substrate at the microscopic level being one of the major restrictions that limits fast, complete cellulose hydrolysis. In the
View All Related Papers

Protocols

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. where is user manual?

    1 answer

    1. This product does not have a protocol associated with it. You may find it helpful to view the peer reviewed articles that mention the use of this product: https://www.sigmaaldrich.com/search/72068?focus=papers&page=1&perpage=30&sort=relevance&term=72068&type=citation_search.

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