This product is prepared according to Pappenheim. The formulation contains approximately 0.62% Eosin-Methylene blue, 0.7% water, and 98.4% methanol.
63590
May-Grünwald solution
Synonym(s):
Eosin – methylene blue solution, Eosin methylene blue according to May-Grünwald
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About This Item
Pricing and availability is not currently available.
technique(s)
microbe id | staining: suitable
Quality Segment
impurities
methanol
density
0.80 g/mL at 20 °C
suitability
Chlamydia spp., Histoplasma spp., Plasmodium spp., Trichomonas spp., bacteria, protozoa, spirochetes
SMILES string
[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
InChI
1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1
InChI key
NXBZXYJXNIFCRJ-UHFFFAOYSA-M
Application
May-Grünwald solution (Eosin – methylene blue solution) is used with Giemsa stain for histological staining of blood cells.
Other Notes
Stain for the panoptic staining according to Pappenheim
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This Item | |||
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| description - | description - | description - | description - |
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) - | technique(s) - |
| suitability spirochetes, Histoplasma spp., Plasmodium spp., Trichomonas spp., Chlamydia spp., protozoa, bacteria | suitability - | suitability - | suitability - |
| Quality Level 100 | Quality Level 500 | Quality Level 500 | Quality Level 500 |
| density 0.80 g/mL at 20 °C | density - | density - | density - |
| impurities methanol | impurities - | impurities - | impurities - |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1
Target Organs
Eyes,Central nervous system
Storage Class
3 - Flammable liquids
WGK
WGK 2
Flash Point (°F)
51.8 °F - closed cup
Flash Point (°C)
11 °C - closed cup
PPE (personal protective equipment)
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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What are the contents of methylene blue, eosin Y, and methanol in this solution?
1 answer-
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What protocol can be used with product 63590?
1 answer-
May Grunwald stain is generally used in combination with Giemsa solution, and there are generally no protocols available for using May Grunwald stain as a stand-alone stain product. The staining obtained with May Grunwald or closely related Romanowsky stain is dependent upon pH, and the pH should be kept within an acceptable range for optimal results.
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