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Atto 647 NHS ester

Name: Atto 647 NHS ester
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

UNSPSC Code:

12352108

NACRES:

NA.25

MDL number:

MFCD05865409

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Properties

product line

BioReagent

Quality Segment

100

assay

≥90% (HPLC), ≥90% (degree of coupling)

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 646-652 nm A_max

suitability

suitable for fluorescence

storage temp.

−20°C

Description

Application

Atto 647 NHS ester is a reagent for the covalent labeling of molecules with Atto 647 flourescent dye. Atto 647 belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, good water solubility, and very little triplet formation. Atto 647 is a zwitterionic dye with a net electrical charge of zero. Atto 647 is a pH sensitive product. While practically stable up to pH 8.5, it slowly degrades at higher pH.

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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This Item	00379	53404	18373
Sigma-Aldrich 07376 Atto 647 NHS ester	Sigma-Aldrich 00379 Atto 495 NHS ester	SAFC 53404 Atto 465 NHS ester	Sigma-Aldrich 18373 Atto 647N NHS ester
description BioReagent, suitable for fluorescence, ≥90% (HPLC)	description BioReagent, suitable for fluorescence, ≥90% (HPLC)	description BioReagent, suitable for fluorescence, ≥90% (HPLC)	description BioReagent, suitable for fluorescence, ≥90% (HPLC)
suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence	suitability suitable for fluorescence
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 100
assay ≥90% (degree of coupling), ≥90% (HPLC)	assay ≥90% (HPLC), ≥90% (degree of coupling)	assay ≥90% (HPLC), ≥90% (degree of coupling)	assay ≥90% (HPLC), ≥90% (degree of coupling)
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
product line BioReagent	product line BioReagent	product line BioReagent	product line BioReagent
UV absorption λ: 646-652 nm A_max	UV absorption λ: 496-502 nm A_max	UV absorption λ: 454-460 nm A_max	UV absorption λ: 641-647 nm A_max

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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