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96351

Supelco

Pullulan Standard Set Mp ~350 - 700′000

set of analytical standards, for GPC

Synonym(s):

Pullulan Standard Set

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About This Item

CAS Number:
9057-02-7
EC Number:
232-945-1
MDL number:
MFCD00027321
UNSPSC Code:
41116107
E Number:
E1204
NACRES:
NA.24
Pricing and availability is not currently available.

grade

analytical standard
for GPC

Quality Level

100

shelf life

limited shelf life, expiry date on the label

analyte chemical class(es)

oligosaccharides

technique(s)

gel permeation chromatography (GPC): suitable

application(s)

cleaning products
cosmetics
food and beverages
personal care

format

neat

storage temp.

2-8°C

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General description

Pullulan is a linear glucan polysaccharide made up of α-1,4-maltotriose repeating units. It is produced by yeast-like fungus Aureobasidium pullulans from starch and has α-1,6 linkages between two maltotriose units.[1]

Application

The set of analytical standards can also be used as follows:
  • Calibration of the polymer-based column in the estimation of molecular weight of the polysaccharide fractions obtained from seaweeds— S. latissima and M. stellatus, by a high-performance size exclusion chromatography (HPSEC) method
  • Measurement of molecular masses of oligomeric cellulose by size exclusion chromatography in combination with multi-angle laser light scattering (MALLS) detection
  • To calibrate the SEC column in the determination of molecular size distribution of microalgal native glycogen
  • Quantitative analysis of membrane formation process of alginate–chitosan microcapsules using gel permeation chromatography(GPC).[2]

Components

Kit with carefully selected reference materials to calibrate GPC/SEC/GFC systems. The kit contains standards of the same type, but with different molar masses, a certificate of analysis (CoA) for each standard and an example calibration curve with analytical conditions.
The standards need to be weighed before use. Suitable for calibrations in aqueous solutions and DMSO.
Kit contains 10 different pullulan standards, each 100 mg, with Mp ranging from ~350 to ~700,000.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCM6880
BCCL0102
BCCK1823
BCCJ8040
BCCJ3796

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Quantitative characterization of membrane formation process of alginate?chitosan microcapsules by GPC
Yu W, et al.
Journal of Membrane Science, 346, 296-301 (2010)
Masako Fujioka-Kobayashi et al.
Biomaterials, 33(30), 7613-7620 (2012-07-18)
To create a drug delivery system that allows the controlled release of proteins, such as growth factors, over a long-term period, cholesteryl group- and acryloyl group-bearing pullulan (CHPOA) nanogels were aggregated to form fast-degradable hydrogels (CHPOA/hydrogels) by cross-linking with thiol-bearing
Jeong-Hun Kang et al.
Journal of controlled release : official journal of the Controlled Release Society, 163(2), 119-124 (2012-09-15)
Apolipoprotein B (Apo B) is a key amphipathic glycoprotein compound in the metabolism of plasma lipoproteins (mainly very low-density lipoprotein (VLDL) and LDL). Inhibition of Apo B synthesis by short interfering RNA (siRNA) targeting Apo B (Apo B siRNA) is
Mehmet Koç et al.
Journal of food science, 76(9), S508-S515 (2012-03-16)
The addition of gelatine (G), lactose (L), pullulan (P), and their mixtures at equal ratios (gelatine + lactose [GL] and gelatin + pullulan [GP]) to whole egg prior to drying was studied. Their effects on the functional properties (emulsion and
Shengjun Wu et al.
International journal of biological macromolecules, 55, 254-257 (2013-02-05)
Pullulan is a thickener that can form semipermeable films, and glutathione is an effective reducing agent, while chitooligosaccharide has antibacterial activity. In this study, effect of pullulan-based coatings in combination with antibrowning and antibacterial agents (1% pullulan; 0.8% glutathione+1% chitooligosaccharides;
View All Related Papers

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Dextran and Polysaccharides Overview

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Questions

  1. What is specification for GPC pullulan standards. Pullulan standard with Mp 22000Da supplied in different lot can vary how much % from 22000Da

    1 answer

    1. This product is intended for use as a calibration standard for GPC/SEC/GFC systems. While there is not specification for the Mp values, these are reported on the lot specific Certificate of Analysis. The lowest value reported for the Mp 220000Da is 19.4000Da. Please see the link below to review a sample or lot specific Certificate:

      https://www.sigmaaldrich.com/product/sial/96351#product-documentation

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