11699113001

MgCl₂ Stock Solution

pH 8.25-8.45 (25 °C), suitable for PCR, solution, pkg of 3 × 1 mL

• Synonym(s): magnesium chloride solution
• UNSPSC Code: 41116100

Properties

• form: solution
• Quality Level: 100
• packaging: pkg of 3 × 1 mL
• manufacturer/tradename: Roche
• concentration: 25 mM
• technique(s): PCR: suitable
• pH: 8.25-8.45 (25 °C)
• storage temp.: −20°C

Description

General description

The MgCl₂ Stock Solution is used for the individual adjustment of the Mg²⁺-concentration in the PCR reaction and can be used in combination with the PCR buffer without MgCl₂. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and the yield of the PCR reaction.

Application

The combination of PCR Buffer without MgCl₂ and the MgCl₂ Stock Solution buffer are optimized for the amplification of specific DNA fragments in conventional PCRs (i.e., with Taq DNA Polymerase). However, the MgCl₂ Stock Solution may be used with other, more appropriate PCR buffers to optimize any type of PCR (e.g., hot start PCR, high fidelity PCR, long template PCR).

Biochem/physiol Actions

Magnesium (Mg²⁺) chelates nucleotide triphosphates (dNTPs) and its usage is one among the optimization strategies for polymerase chain reaction (PCR).[1] It promotes orthophosphate synthesis from oligophosphates by stabilizing the phosphate groups of nucleotides.[2]

Physical form

The MgCl₂ Stock Solution contains 25mM MgCl₂ in sterile water.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): does not flash
• Flash Point(C): does not flash