SCT031

BioTracker 515 Green Zn2+ Dye

Live cell imaging dye for zinc (Zn2+) for cell signaling, protein self-assembly and cellular metabolism applications.

• Synonym(s): Live cell imaging probe
• UNSPSC Code: 12352207
• NACRES: NA.47
• eCl@ss: 32161000

Properties

• technique(s): cell based assay: suitable, live cell imaging : suitable
• detection method: fluorometric
• shipped in: ambient
• storage temp.: -10 to -25°C

Description

General description

Zinc (Zn2+), is a trace nutrient critical for physiological function. Although most biological zinc ions are tightly associated with proteins, pools of loosely bound or mobile forms serve regulatory or signaling functions, including nucleation of protein self-assembly, triggering of signaling pathways, and modification of cellular metabolism.

The BioTracker 515 Green Zn2+ Dye is a fluorescent imaging probe for the detection of Zn2+ in living cells. The BioTracker 515 Green Zn2+ Dye has high solubility, so that it is not permeable through the cell membrane. BioTracker 515 Green Zn2+ Dye DA has an additional acetyl group which make the reagent go inside the cell much easier, and the acetyl group is removed by the intracellular esterase which make the reagent stay inside the cell for extended periods of time.

Concentration of the provided sample is 5 mmol/L in DMSO. Dilute 1000 times with neutral buffer before use.

Spectral Properties
Absorbance: 492nm
Emission: 515nm

Preparation Note

Store BioTracker 515 Green Zn2+ Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): 188.6 °F - Solvent
• Flash Point (°C): 87 °C - Solvent