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GF05

Sigma-Aldrich

Anti-Granulocyte Colony Stimulating Factor (Ab-1) Mouse mAb (5.24)

liquid, clone 5.24, Calbiochem®

Synonym(s):

Anti-G-CSF

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

5.24, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

do not freeze

isotype

IgG2

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CSF3(1440)

General description

Anti-Granulocyte Colony Stimulating Factor (Ab-1) mouse monoclonal, clone 5.24, recognizes ~19 kDa G-CSF in bone marrow & fetal liver tissue. It is validated for use in WB, IF & IHC (paraffin).
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes granulocyte colony stimulating factor.
Recognizes the ~19 kDa G-CSF glycoprotein in bone marrow and fetal liver tissue.

Immunogen

Human
purified human G-CSF

Application

Immunoblotting (1-5 µg/ml)

Immunofluorescence (1-5 µg/ml)

Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
Recombinant G-CSF protein, bone marrow tissue, or fetal liver tissue

Other Notes

For paraffin sections, saponin treatment is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.
Nicola, N.A. 1987. J. Cell Cloning5, 1.
Metcalf, D., 1986. Blood67, 257.
Nagata, S., et al. 1986. Nature319, 415.
Nagata, S., et al. 1986 EMBO J.5, 575.
Souza, L.M., et al. 1986 Science232, 61.
Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.
Nicola, N.A. Vadas, M.A., 1984. Immunol Today5, 76.
Nicola, N.A., et al. 1983 J. Biol. Chem.258, 9017.
Burgess, A.W., Metcalf, D. 1980. J. Cancer26, 647.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kazuhide Shimamatsu et al.
Oncology letters, 15(2), 2387-2392 (2018-02-13)
The present case study documents an autopsy case of granulocyte-colony stimulating factor (G-CSF)-producing mucinous cystic neoplasm (MCN), with an associated invasive carcinoma of the pancreas. A 65-year-old woman presented to Omuta City Hospital (Omuta Japan) with a primary complaint of

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