AB9712

Anti-Sortilin Antibody

serum, Chemicon^®

• Synonym(s): Neurotensin receptor 3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 2

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NM_002959.4
• UniProt accession no.: Q99523
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... SORT1(6272)

Description

General description

Neurotensin receptor 3 or sortilin (the intracellular sorting protein 95 kDa) is a member of a family of receptors characterized by a single transmembrane domain, a short intracytoplasmic domain, and a long cysteine-rich luminal extracellular domain. This receptor has been found in the brain, spinal cord, muscle and other peripheral cell types, and in cancer cells. Sortilin exhibits sequence similarities with proteins involved in intracellular transport and trafficking.

Immunogen

Recombinant protein from the extracellular domain of human sortilin.

Application

Anti-Sortilin Antibody is an antibody against Sortilin for use in WB, IH.

Western blot: 1:1,000-1:2,000 on extracts from NSC34 cells.
Immunohistochemistry: 1:500-1:1,000 on rat spinal cord.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Sortilin (Neurotensin receptor 3). By Western blot the antibody recognizes a strong band at ~90 kDa in extracts of NSC34 cells. By immunohistochemistry the antibody intensely stains motor neurons within the rat spinal cord.

Physical form

Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material.

Preparation Note

Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added 1:1 for additional stability.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable